Soil microbes and nutrient inputs influence root nodulation and tree performance in Alnus glutinosa

Applied Soil Ecology, vol. 215, pp. 106466

Abstract

The symbiotic relationship between the nitrogen-fixing bacteria Frankia alni and the pioneer tree species Alnus glutinosa plays an important role for tree performance, helping trees thrive in nitrogen-poor environments. However, F. alni nodulation can vary greatly between different soils and this could be due to biotic and abiotic characteristics of the soil. Here we examine how microbial communities from young (∼10 years) and mature (≥100 years) forests and gradients of available nitrogen (N) and phosphorus (P) influence F. alni nodulation and tree performance. In mesocosm experiments, A. glutinosa seedlings were inoculated with bacteria, fungi, or both, cultured from young and mature forest soils, alongside F. alni . The impacts of N and P availability were examined through controlled nutrient manipulations. Results demonstrated that fungal communities from mature forests suppressed the growth-promoting effects of F. alni , although nodule biomass itself was not directly influenced. Further, we isolated and identified bacteria and fungi that were found to contribute to the observed inhibitory effects on F. alni -mediated growth promotion. Increased N availability significantly reduced nodule biomass, and we established a threshold at which reliance on symbiosis diminished. Conversely, P-addition stimulated nodulation and tree growth. These findings highlight the pivotal influence of N and P availability in determining the A. glutinosa-F. alni symbiosis, while also providing evidence that specific microbes in the soil influence these dynamics. • Microbes from young and mature forests did not directly affect nodulation. • Fungi from mature forests suppress the growth promoting effects of F. alni . • Specific bacteria and fungi were isolated that contribute to the suppressive effects. • Nitrogen suppressed root nodulation while phosphorus stimulated it.

Authors 6

1. Konstantinos Georgopoulos corresponding Aachen Above-Belowground Interactions Group Institute of Biology, Leiden University

   Leiden University · University of Copenhagen

   Affiliation as printed

   Above-Belowground Interactions Group, Institute of Biology, Leiden University, Sylviusweg, Leiden, the Netherlands

   Department of Geosciences and Natural Resource Management, University of Copenhagen, Rolighedsvej, Denmark

2. Т. Martijn Bezemer Aachen Above-Belowground Interactions Group Institute of Biology, Leiden University

   Leiden University

   Affiliation as printed

   Above-Belowground Interactions Group, Institute of Biology, Leiden University, Sylviusweg, Leiden, the Netherlands

3. Lars Vesterdal

   University of Copenhagen

   Affiliation as printed

   Department of Geosciences and Natural Resource Management, University of Copenhagen, Rolighedsvej, Denmark

4. Léon de Nobel Aachen Above-Belowground Interactions Group Institute of Biology, Leiden University

   Leiden University

   Affiliation as printed

   Above-Belowground Interactions Group, Institute of Biology, Leiden University, Sylviusweg, Leiden, the Netherlands

5. Naksha Kasal Aachen Above-Belowground Interactions Group Institute of Biology, Leiden University

   Leiden University

   Affiliation as printed

   Above-Belowground Interactions Group, Institute of Biology, Leiden University, Sylviusweg, Leiden, the Netherlands

6. Sofia I. F. Gomes Aachen Above-Belowground Interactions Group Institute of Biology, Leiden University

   Leiden University

   Affiliation as printed

   Above-Belowground Interactions Group, Institute of Biology, Leiden University, Sylviusweg, Leiden, the Netherlands