Pathophysiological response in trauma-related acute kidney injury after blunt thoracic trauma and hemorrhagic shock in male mice

American Journal of Physiology-Renal Physiology, vol. 329, pp. F311–F322

Abstract

This study introduces a murine model of trauma-related acute kidney injury (TRAKI) via combined blunt thoracic trauma and hemorrhage, revealing transient kidney dysfunction despite normal morphology. Early damage to proximal tubular cells, inflammatory responses, and induction of stress markers like heme oxygenase-1 were observed. Proteomic analyses uncovered distinct intrarenal changes, whereas three-dimensional microcomputed tomography showed capillary blood supply impairment, resolving by day 5. These findings shed light on TRAKI’s pathophysiology and may inform future therapeutic strategies.

Authors 16

1. Rebecca Halbgebauer

   Universität Ulm · University Hospital Ulm

   Affiliation as printed

   Ulm University Medical Center

   Institute of Clinical and Experimental Trauma Immunology, Ulm University Medical Center, Ulm, Germany

2. Lorena Schult

   Universität Ulm · University Hospital Ulm

   Affiliation as printed

   Ulm University Medical Center

   Institute of Clinical and Experimental Trauma Immunology, Ulm University Medical Center, Ulm, Germany

3. Onno Borgel

   Universität Ulm · University Hospital Ulm

   Affiliation as printed

   Ulm University Medical Center

   Institute of Clinical and Experimental Trauma Immunology, Ulm University Medical Center, Ulm, Germany

4. Arne Maes

   KU Leuven · UCLouvain · Scientific Research Institute of Experimental and Clinical Medicine

   Affiliation as printed

   Institute of Experimental and Clinical Research, UCLouvain

   Institute of Mechanics, Materials and Civil Engineering, UCLouvain

   KU Leuven

   Biomechanics Lab, Institute of Mechanics, Materials and Civil Engineering, UCLouvain, Louvain-la-Neuve, Belgium

   Department of Materials Engineering, KU Leuven, Heverlee, Belgium

   Institute of Experimental and Clinical Research

   Pole of Morphology, Institute of Experimental and Clinical Research, UCLouvain, Brussels, Belgium

5. Florian Weißhaupt

   Universität Ulm · University Hospital Ulm

   Affiliation as printed

   Ulm University Medical Center

   Institute of Clinical and Experimental Trauma Immunology, Ulm University Medical Center, Ulm, Germany

6. Christina Rastner

   Universität Ulm · University Hospital Ulm

   Affiliation as printed

   Ulm University Medical Center

   Institute of Clinical and Experimental Trauma Immunology, Ulm University Medical Center, Ulm, Germany

7. Alitsia Ast

   Universität Ulm · University Hospital Ulm

   Affiliation as printed

   Ulm University Medical Center

   Institute of Clinical and Experimental Trauma Immunology, Ulm University Medical Center, Ulm, Germany

8. Ludmila Lupu

   Universität Ulm · University Hospital Ulm

   Affiliation as printed

   Ulm University Medical Center

   Institute of Clinical and Experimental Trauma Immunology, Ulm University Medical Center, Ulm, Germany

9. Annette Palmer

   Universität Ulm

   Affiliation as printed

   Ulm University Medical Center

   University of Ulm

   Molecular Cardiology, Department of Internal Medicine II, University of Ulm, Ulm, Germany

10. Ulrich A. Wachter

    Universität Ulm

    Affiliation as printed

    University of Ulm

    Institute for Anaesthesiological Pathophysiology and Process Development, University of Ulm, Ulm, Germany

11. Stefan Andreas Schmidt

    Universität Ulm · University Hospital Ulm

    Affiliation as printed

    Ulm University Medical Center

    Clinic for Diagnostic and Interventional Radiology, Ulm University Medical Center, Ulm, Germany

12. Peter Boor Aachen Institute of Pathology

    RWTH Aachen University · Universitätsklinikum Aachen

    Affiliation as printed

    RWTH Aachen University Hospital

    Institute of Pathology, RWTH Aachen University Hospital, Aachen, Germany

13. Reinhild Rösler

    Universität Ulm

    Affiliation as printed

    Core Unit Mass Spectrometry and Proteomics, Ulm University

    Core Unit Mass Spectrometry and Proteomics, Ulm University, Ulm, Germany

    Ulm University

14. Sebastian Wiese

    Universität Ulm

    Affiliation as printed

    Core Unit Mass Spectrometry and Proteomics, Ulm University

    Core Unit Mass Spectrometry and Proteomics, Ulm University, Ulm, Germany

    Ulm University

15. Greet Kerckhofs

    KU Leuven · UCLouvain · Scientific Research Institute of Experimental and Clinical Medicine

    Affiliation as printed

    Institute of Experimental and Clinical Research, UCLouvain

    Institute of Mechanics, Materials and Civil Engineering, UCLouvain

    KU Leuven

    Biomechanics Lab, Institute of Mechanics, Materials and Civil Engineering, UCLouvain, Louvain-la-Neuve, Belgium

    Department of Materials Engineering, KU Leuven, Heverlee, Belgium

    Division for Skeletal Tissue Engineering

    Institute of Experimental and Clinical Research

    Pole of Morphology, Institute of Experimental and Clinical Research, UCLouvain, Brussels, Belgium

16. Markus Stefan Huber-Lang corresponding

    Universität Ulm · University Hospital Ulm

    Affiliation as printed

    Ulm University Medical Center

    Institute of Clinical and Experimental Trauma Immunology, Ulm University Medical Center, Ulm, Germany