Constraint-based modeling of bioenergetic differences between synaptic and non-synaptic components of dopaminergic neurons in Parkinson’s disease

Frontiers in Computational Neuroscience, vol. 19, pp. 1594330

Abstract

Introduction: Emerging evidence suggests that different metabolic characteristics, particularly bioenergetic differences, between the synaptic terminal and soma may contribute to the selective vulnerability of dopaminergic neurons in patients with Parkinson's disease (PD). Method: To investigate the metabolic differences, we generated four thermodynamically flux-consistent metabolic models representing the synaptic and non-synaptic (somatic) components under both control and PD conditions. Differences in bioenergetic features and metabolite exchanges were analyzed between these models to explore potential mechanisms underlying the selective vulnerability of dopaminergic neurons. Bioenergetic rescue analyses were performed to identify potential therapeutic targets for mitigating observed energy failure and metabolic dysfunction in PD models. Results: All models predicted that oxidative phosphorylation plays a significant role under lower energy demand, while glycolysis predominates when energy demand exceeds mitochondrial constraints. The synaptic PD model predicted a lower mitochondrial energy contribution and higher sensitivity to Complex I inhibition compared to the non-synaptic PD model. Both PD models predicted reduced uptake of lysine and lactate, indicating coordinated metabolic processes between these components. In contrast, decreased methionine and urea uptake was exclusively predicted in the synaptic PD model, while decreased histidine and glyceric acid uptake was exclusive to the non-synaptic PD model. Furthermore, increased flux of the mitochondrial ornithine transaminase reaction (ORNTArm), which converts oxoglutaric acid and ornithine into glutamate-5-semialdehyde and glutamate, was predicted to rescue bioenergetic failure and improve metabolite exchanges for both the synaptic and non-synaptic PD models. Discussion: The predicted differences in ATP contribution between models highlight the bioenergetic differences between these neuronal components, thereby contributing to the selective vulnerability observed in PD. The observed differences in metabolite exchanges reflect distinct metabolic patterns between these neuronal components. Additionally, mitochondrial ornithine transaminase was predicted to be the potential bioenergetic rescue target for both the synaptic and non-synaptic PD models. Further research is needed to validate these dysfunction mechanisms across different components of dopaminergic neurons and to explore targeted therapeutic strategies for PD patients.

Authors 5

1. Xi Luo

   Ollscoil na Gaillimhe – University of Galway

   Affiliation as printed

   School of Medicine, University of Galway, Galway, Ireland

2. Diana Charles El Assal

   New York University Abu Dhabi

   Affiliation as printed

   Division of Science and Math, New York University Abu Dhabi, Abu Dhabi, United Arab Emirates

3. Yanjun Liu

   Ollscoil na Gaillimhe – University of Galway

   Affiliation as printed

   School of Medicine, University of Galway, Galway, Ireland

4. Samira Ranjbar

   Ollscoil na Gaillimhe – University of Galway

   Affiliation as printed

   School of Medicine, University of Galway, Galway, Ireland

5. Ronan M. T. Fleming corresponding Aachen Division of Systems Biomedicine and Pharmacology Leiden Academic Centre for Drug Research

   Leiden University · Ollscoil na Gaillimhe – University of Galway

   Affiliation as printed

   Division of Systems Biomedicine and Pharmacology, Leiden Academic Center for Drug Research, Leiden University, Leiden, Netherlands

   School of Medicine, University of Galway, Galway, Ireland