Composition variations in archaeological human bone proteomes

Journal of Archaeological Science, vol. 190, pp. 106560

Abstract

Abstract Sampling strategies within the field of skeletal palaeoproteomics are often based on specimen availability. Knowledge of bone biology might assist in improving sample selection strategies and minimise unnecessary sampling of precious (hominin) material. We study ten bone sample locations across four bone elements, for a total of 10 adult, archaeological human skeletons. We compare bone proteome composition and modification for skeletal elements formed through endochondral and intramembranous ossification, as well as cortical-trabecular bone pairs of three skeletal locations. We observe minimal differences in bones formed through the two ossification processes, outside of the exclusive presence of cartilage-related proteins in endochondral bone samples. We observe higher protein concentrations, a larger number of protein groups and peptides, and lower rates of deamidation in cortical bone compared to trabecular bone proteomes, this indicates that cortical bone provides a better preservation environment compared to trabecular bone. Throughout our analysis, the petrous bone stands out, with the largest and most complex proteomes recovered for all studied individuals. Formed through endochondral ossification, the petrous bone undergoes minimal turnover during life. Our observations indicate that the petrous bone is the ideal source of ancient protein sequence information.

Authors 5

1. Ragnheiður Diljá Ásmundsdóttir corresponding

   University of Copenhagen

   Affiliation as printed

   Globe Institute, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

2. Gaudry Troché

   University of Copenhagen

   Affiliation as printed

   Globe Institute, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

3. Jesper Velgaard Olsen

   University of Copenhagen · Novo Nordisk Foundation

   Affiliation as printed

   Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

4. Sarah A. Schrader Aachen Faculty of Archaeology, Leiden University

   Leiden University

   Affiliation as printed

   Faculty of Archaeology, Leiden University, Leiden, the Netherlands

5. Frido Welker corresponding

   University of Copenhagen

   Affiliation as printed

   Globe Institute, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark