Effect of a 24-week supervised concurrent exercise intervention on fecal microbiota diversity and composition in young sedentary adults: The ACTIBATE randomized controlled trial
Clinical Nutrition, vol. 49, pp. 128–137
Abstract
BACKGROUND: Numerous physiological responses to exercise are observed in humans, yet the effects of long-term exercise and varying intensities on the diversity and composition of human fecal microbiota remain unclear. We investigated the effect of a 24-week supervised concurrent exercise intervention, at moderate and vigorous intensities, on fecal microbiota diversity and composition in young adults. METHODS: This ancillary study was based on data from the ACTIBATE randomized controlled trial (ClinicalTrials.gov ID: NCT02365129), and included adults (aged 18-25 years, 70 % female) that were randomized to (i) a control group (CON: no exercise, n = 20), (ii) a moderate-intensity exercise group (MOD-EX, n = 21), and (iii) a vigorous-intensity exercise group (VIG-EX, n = 20). Fecal samples were collected before and after the 24-week exercise intervention, and the diversity and composition of the fecal microbiota were analyzed by 16S rRNA sequencing. Inferential functional profiling of the fecal microbiota was performed and correlations between microbial changes and cardiometabolic outcomes were assessed. RESULTS: Exercise did not modify beta or alpha diversities regardless of the intensity (all P ≥ 0.062). The relative abundance of the Erysipelotrichaceae family (Bacillota phylum) (-0.3 ± 1.2 %; P = 0.031) was however reduced in the VIG-EX group. Coprococcus was the only genus showed a significant difference between MOD-EX and VIG-EX after the intervention, with its relative abundance increasing in MOD-EX (+0.4 ± 0.6 %; P = 0.005). None of these changes were related to the exercise-induced cardiometabolic benefits (all P ≥ 0.05). CONCLUSIONS: In young adults, a 24-week supervised concurrent exercise program, at moderate and vigorous intensities, resulted in minor changes in fecal microbiota composition, while neither alpha nor beta diversities were affected. CLINICAL TRIAL REGISTRATION: ClinicalTrials.gov ID: NCT02365129.
Authors 19
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Leiden University · Universidad de Granada · Leiden University Medical Center · University of Almería
Affiliation as printed
Department of Physical Education and Sports, Faculty of Sports Science, Sport and Health University Research Institute (iMUDS), University of Granada, 18071, Granada, Spain; Department of Medicine, Division of Endocrinology, and Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, 2333 ZA, Leiden, the Netherlands; CIBEROBN, Biomedical Research Networking Center for Physiopathology of Obesity and Nutrition, Carlos III Health Institute, 18100, Granada, Spain; Department of Nursing, Physiotherapy and Medicine and SPORT Research Group, CIBIS Research Center, University of Almería, 04120, Almería, Spain. Electronic address: borjammt@ual.es
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Affiliation as printed
Department of Physical Education and Sports, Faculty of Sports Science, Sport and Health University Research Institute (iMUDS), University of Granada, 18071, Granada, Spain; Department of Biochemistry and Molecular Biology II, School of Pharmacy, University of Granada, 1807, Granada, Spain
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Affiliation as printed
Department of Physical Education and Sports, Faculty of Sports Science, Sport and Health University Research Institute (iMUDS), University of Granada, 18071, Granada, Spain; Department of Biochemistry and Molecular Biology II, School of Pharmacy, University of Granada, 1807, Granada, Spain
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Tufts University · Jean Mayer Human Nutrition Research Center on Aging
Affiliation as printed
Vitamins and Carcinogenesis Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA, 02111, USA
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Milena Schönke Aachen Department of Medicine Division of Endocrinology, and Einthoven Laboratory for Experimental Vascular Medicine
Leiden University · Leiden University Medical Center
Affiliation as printed
Department of Medicine, Division of Endocrinology, and Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, 2333 ZA, Leiden, the Netherlands
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Affiliation as printed
Department of Physical Education and Sports, Faculty of Sports Science, Sport and Health University Research Institute (iMUDS), University of Granada, 18071, Granada, Spain; Department of Physiology, Faculty of Medicine, University of Granada, Av. Conocimiento s/n, 18011, Granada, Spain
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Affiliation as printed
Department of Physical Education and Sports, Faculty of Sports Science, Sport and Health University Research Institute (iMUDS), University of Granada, 18071, Granada, Spain; Department of Analytical Chemistry, University of Granada, 18071, Granada, Spain
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Universidad de Granada · Universidad Pública de Navarra (UPNA) · Instituto de Innovación y Sostenibilidad en la Cadena Agroalimentaria · Universidad de Navarra
Affiliation as printed
Department of Physical Education and Sports, Faculty of Sports Science, Sport and Health University Research Institute (iMUDS), University of Granada, 18071, Granada, Spain; Institute for Innovation & Sustainable Development in Food Chain Development (IS-FOOD), Department of Health Sciences, Public University of Navarra, Campus de Arrosadía, 31006, Pamplona, Spain
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University of Turku · Universidad de Granada · Turku University Hospital · Turku PET Centre
Affiliation as printed
Department of Physical Education and Sports, Faculty of Sports Science, Sport and Health University Research Institute (iMUDS), University of Granada, 18071, Granada, Spain; Turku PET Centre, University of Turku, Turku, Finland; Turku PET Centre, Turku University Hospital, 20520, Turku, Finland
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Universidad de Granada · Instituto de Salud Carlos III · Instituto de Investigación Biosanitaria de Granada
Affiliation as printed
Department of Physical Education and Sports, Faculty of Sports Science, Sport and Health University Research Institute (iMUDS), University of Granada, 18071, Granada, Spain; CIBEROBN, Biomedical Research Networking Center for Physiopathology of Obesity and Nutrition, Carlos III Health Institute, 18100, Granada, Spain; Department of Physiology, Faculty of Medicine, University of Granada, Av. Conocimiento s/n, 18011, Granada, Spain; Instituto de Investigación Biosanitaria, ibs.Granada, 18012, Granada, Spain
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Affiliation as printed
Division of Pharmacology, Faculty of Science, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 3584 CG, Utrecht, the Netherlands
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Ludwig-Maximilians-Universität München
Affiliation as printed
Medical Department II, University Hospital, Ludwig-Maximilians-Universität, 80336, Munich, Germany
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Otto-von-Guericke-Universität Magdeburg
Affiliation as printed
Department of Gastroenterology, Hepatology and Infectious Diseases, Otto-von-Guericke-University Magdeburg, 39120, Magdeburg, Germany
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Universidad de Granada · Instituto de Investigación Biosanitaria de Granada · Universidad Internacional de La Rioja
Affiliation as printed
Department of Biochemistry and Molecular Biology II, School of Pharmacy, University of Granada, 1807, Granada, Spain; Instituto de Investigación Biosanitaria, ibs.Granada, 18012, Granada, Spain; School of Health Sciences, Universidad Internacional de La Rioja, Avenida de la Paz, 137, 26006, Logroño, Spain
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Universidad de Granada · Instituto de Salud Carlos III
Affiliation as printed
CIBEROBN, Biomedical Research Networking Center for Physiopathology of Obesity and Nutrition, Carlos III Health Institute, 18100, Granada, Spain; Department of Biochemistry and Molecular Biology II, School of Pharmacy, University of Granada, 1807, Granada, Spain; Institute of Nutrition and Food Technology "José Mataix", Biomedical Research Center, Parque Tecnológico Ciencias de la Salud, University of Granada, Armilla, 18016, Granada, Spain
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Universidad Pública de Navarra (UPNA) · Instituto de Salud Carlos III · Instituto de Innovación y Sostenibilidad en la Cadena Agroalimentaria · Universidad de Navarra
Affiliation as printed
CIBEROBN, Biomedical Research Networking Center for Physiopathology of Obesity and Nutrition, Carlos III Health Institute, 18100, Granada, Spain; Institute for Innovation & Sustainable Development in Food Chain Development (IS-FOOD), Department of Health Sciences, Public University of Navarra, Campus de Arrosadía, 31006, Pamplona, Spain
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Instituto de Salud Carlos III · Centro de Investigación Biomédica en Red Diabetes y Enfermedades Metabólicas Asociadas · Institut de Recerca Biomèdica Catalunya Sud · Hospital Universitari Joan XXIII de Tarragona · Universitat Rovira i Virgili
Affiliation as printed
Hospital Universitari Joan XXIII de Tarragona, Institut d'Investigació Sanitària Pere Virgili (IISPV), 43204, Tarragona, Spain; CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM)-Instituto de Salud Carlos III (ISCIII), 28029, Madrid, Spain; Universitat Rovira i Virgili, 43003, Tarragona, Spain
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Patrick C.N. Rensen Aachen Department of Medicine Division of Endocrinology, and Einthoven Laboratory for Experimental Vascular Medicine
Leiden University · Leiden University Medical Center
Affiliation as printed
Department of Medicine, Division of Endocrinology, and Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, 2333 ZA, Leiden, the Netherlands
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Jonatan R. Ruiz corresponding
Universidad de Granada · Instituto de Salud Carlos III · Instituto de Investigación Biosanitaria de Granada
Affiliation as printed
Department of Physical Education and Sports, Faculty of Sports Science, Sport and Health University Research Institute (iMUDS), University of Granada, 18071, Granada, Spain; CIBEROBN, Biomedical Research Networking Center for Physiopathology of Obesity and Nutrition, Carlos III Health Institute, 18100, Granada, Spain; Instituto de Investigación Biosanitaria, ibs.Granada, 18012, Granada, Spain. Electronic address: ruizj@ugr.es
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