The IBEX Imaging Knowledge-Base: A Community Resource Enabling Adoption and Development of Immunofluoresence Imaging Methods
Abstract
Abstract The iterative bleaching extends multiplexity (IBEX) Knowledge-Base is a central portal for researchers adopting IBEX and related 2D and 3D immunofluorescence imaging methods. The design of the Knowledge-Base is modeled after efforts in the open-source software community and includes three facets: a development platform (GitHub), static website, and service for data archiving. The Knowledge-Base facilitates the practice of open science throughout the research life cycle by providing validation data for recommended and non-recommended reagents, e.g., primary and secondary antibodies. In addition to reporting negative data, the Knowledge-Base empowers method adoption and evolution by providing a venue for sharing protocols, videos, datasets, software, and publications. A dedicated discussion forum fosters a sense of community among researchers while addressing questions not covered in published manuscripts. Together, scientists from around the world are advancing scientific discovery at a faster pace, reducing wasted time and effort, and instilling greater confidence in the resulting data.The iterative bleaching extends multiplexity (IBEX) Knowledge-Base is a central portal for researchers adopting IBEX and related 2D and 3D immunofluorescence imaging methods. The design of the Knowledge-Base is modeled after efforts in the open-source software community and includes three facets: a development platform (GitHub), static website, and service for data archiving. The Knowledge-Base facilitates the practice of open science throughout the research life cycle by providing validation data for recommended and non-recommended reagents, e.g., primary and secondary antibodies. In addition to reporting negative data, the Knowledge-Base empowers method adoption and evolution by providing a venue for sharing protocols, videos, datasets, software, and publications. A dedicated discussion forum fosters a sense of community among researchers while addressing questions not covered in published manuscripts. Together, scientists from around the world are advancing scientific discovery at a faster pace, reducing wasted time and effort, and instilling greater confidence in the resulting data.The iterative bleaching extends multiplexity (IBEX) Knowledge-Base is a central portal for researchers adopting IBEX and related 2D and 3D immunofluorescence imaging methods. The design of the Knowledge-Base is modeled after efforts in the open-source software community and includes three facets: a development platform (GitHub), static website, and service for data archiving. The Knowledge-Base facilitates the practice of open science throughout the research life cycle by providing validation data for recommended and non-recommended reagents, e.g., primary and secondary antibodies. In addition to reporting negative data, the Knowledge-Base empowers method adoption and evolution by providing a venue for sharing protocols, videos, datasets, software, and publications. A dedicated discussion forum fosters a sense of community among researchers while addressing questions not covered in published manuscripts. Together, scientists from around the world are advancing scientific discovery at a faster pace, reducing wasted time and effort, and instilling greater confidence in the resulting data.The iterative bleaching extends multiplexity (IBEX) Knowledge-Base is a central portal for researchers adopting IBEX and related 2D and 3D immunofluorescence imaging methods. The design of the Knowledge-Base is modeled after efforts in the open-source software community and includes three facets: a development platform (GitHub), static website, and service for data archiving. The Knowledge-Base facilitates the practice of open science throughout the research life cycle by providing validation data for recommended and non-recommended reagents, e.g., primary and secondary antibodies. In addition to reporting negative data, the Knowledge-Base empowers method adoption and evolution by providing a venue for sharing protocols, videos, datasets, software, and publications. A dedicated discussion forum fosters a sense of community among researchers while addressing questions not covered in published manuscripts. Together, scientists from around the world are advancing scientific discovery at a faster pace, reducing wasted time and effort, and instilling greater confidence in the resulting data.
Authors 61
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National Institutes of Health · National Institute of Allergy and Infectious Diseases
Affiliation as printed
Bioinformatics and Computational Bioscience Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health
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National Institutes of Health · National Heart, Lung, and Blood Institute
Affiliation as printed
Critical Care Medicine and Pulmonary Branch, National Heart, Lung and Blood Institute, National Institutes of Health
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National Institutes of Health · National Institute of Allergy and Infectious Diseases
Affiliation as printed
Lymphocyte Biology Section, Laboratory of Immune System Biology, NIAID, NIH
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National Institutes of Health · National Institute of Allergy and Infectious Diseases
Affiliation as printed
Lymphocyte Biology Section, Laboratory of Immune System Biology, NIAID, NIH
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National Institutes of Health · National Institute of Allergy and Infectious Diseases
Affiliation as printed
Lymphocyte Biology Section, Laboratory of Immune System Biology, NIAID, NIH
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Affiliation as printed
Department of Intelligent Systems Engineering, Indiana University
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University College London · NIHR Moorfields Biomedical Research Centre
Affiliation as printed
UCL Institute of Ophthalmology and NIHR Moorfields Biomedical Research Centre
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National Institutes of Health · National Institute of Allergy and Infectious Diseases
Affiliation as printed
Lymphocyte Biology Section, Laboratory of Immune System Biology, NIAID, NIH
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University of Cambridge · MRC Laboratory of Molecular Biology · Molecular Immunity Unit
Affiliation as printed
Cambridge Institute for Therapeutic Immunology and Infectious Diseases, University of Cambridge Department of Medicine, Molecular Immunity Unit, Laboratory of Molecular Biology
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Affiliation as printed
McMaster Immunology Research Centre, Schroeder Allergy and Immunology Research Institute, Department of Medicine, Faculty of Health Sciences, McMaster University
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Affiliation as printed
Max-Delbrueck-Center for Molecular Medicine in the Helmholtz Association (MDC), Spatial Proteomics Group
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Affiliation as printed
Department of Business Development, BioLegend Inc
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Affiliation as printed
McMaster Immunology Research Centre, Schroeder Allergy and Immunology Research Institute, Department of Medicine, Faculty of Health Sciences, McMaster University
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National Institutes of Health · National Institute of Arthritis and Musculoskeletal and Skin Diseases
Affiliation as printed
Functional Immunogenomics Unit, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health
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Universidade Federal de Minas Gerais
Affiliation as printed
Laboratory of Cell-Cell Interactions, Department of Morphology, Institute of Biological Sciences, Universidade Federal de Minas Gerais
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Affiliation as printed
Max-Delbrueck-Center for Molecular Medicine in the Helmholtz Association (MDC), Spatial Proteomics Group
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Rush University Medical Center
Affiliation as printed
Division of Rheumatology, Rush University Medical Center
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Affiliation as printed
Department of Immunology, University of Washington School of Medicine
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Affiliation as printed
Robin Chemers Neustein Laboratory of Mammalian Cell Biology and Development, The Rockefeller University
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Hospital Israelita Albert Einstein
Affiliation as printed
Center for Research in Immuno-oncology (CRIO), Hospital Israelita Albert Einstein
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National Institutes of Health · National Cancer Institute
Affiliation as printed
Surgical Oncology Program, National Cancer Institute, National Institutes of Health
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National Institutes of Health · National Institute of Allergy and Infectious Diseases
Affiliation as printed
Lymphocyte Biology Section, Laboratory of Immune System Biology, NIAID, NIH
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National Institutes of Health · National Institute of Allergy and Infectious Diseases
Affiliation as printed
Lymphocyte Biology Section, Laboratory of Immune System Biology, NIAID, NIH
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National Institutes of Health · National Institute of Allergy and Infectious Diseases
Affiliation as printed
Lymphocyte Biology Section, Laboratory of Immune System Biology, NIAID, NIH
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RWTH Aachen University · German Center for Lung Research
Affiliation as printed
German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH)
Institute of Pathology, Aachen Medical University, RWTH Aachen
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National Institutes of Health · National Institute of Allergy and Infectious Diseases
Affiliation as printed
Lymphocyte Biology Section, Laboratory of Immune System Biology, NIAID, NIH
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University of Würzburg · Max Planck Society
Affiliation as printed
Würzburg Institute of Systems Immunology, Max Planck Research Group at the Julius-Maximilians-Universität Würzburg
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Affiliation as printed
McMaster Immunology Research Centre, Schroeder Allergy and Immunology Research Institute, Department of Medicine, Faculty of Health Sciences, McMaster University
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University College London · NIHR Moorfields Biomedical Research Centre
Affiliation as printed
UCL Institute of Ophthalmology and NIHR Moorfields Biomedical Research Centre
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Affiliation as printed
Department of Medicine, McMaster University, Firestone Institute for Respiratory Health, St Joseph’s Healthcare
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Affiliation as printed
Infection Immunity and Inflammation Research and Teaching Department, University College London Great Ormond Street Institute of Child Health
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National Institutes of Health · National Institute of Allergy and Infectious Diseases
Affiliation as printed
Lymphocyte Biology Section, Laboratory of Immune System Biology, NIAID, NIH
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National Institutes of Health · National Cancer Institute
Affiliation as printed
Surgical Oncology Program, National Cancer Institute, National Institutes of Health
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Hospital Israelita Albert Einstein
Affiliation as printed
Center for Research in Immuno-oncology (CRIO), Hospital Israelita Albert Einstein
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National Institutes of Health · National Heart, Lung, and Blood Institute
Affiliation as printed
Critical Care Medicine and Pulmonary Branch, National Heart, Lung and Blood Institute, National Institutes of Health
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Affiliation as printed
Viral Vector Laboratory, Cancer Institute of São Paulo, University of São Paulo
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University College London · NIHR Moorfields Biomedical Research Centre
Affiliation as printed
UCL Institute of Ophthalmology and NIHR Moorfields Biomedical Research Centre
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National Institutes of Health · National Cancer Institute
Affiliation as printed
Neuro-Oncology Branch, National Cancer Institute, National Institutes of Health
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University of California San Diego
Affiliation as printed
Division of Allergy, Immunology and Rheumatology, Department of Pediatrics, University of California San Diego
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Affiliation as printed
Department of Medicine, McMaster University, Firestone Institute for Respiratory Health, St Joseph’s Healthcare
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Northwestern University · Northwestern University
Affiliation as printed
Department of Cellular and Developmental Biology, Northwestern University
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University College London · Universidad Nacional Autónoma de México
Affiliation as printed
Infection Immunity and Inflammation Research and Teaching Department, University College London Great Ormond Street Institute of Child Health
Laboratorio de Bioingeniería de Tejidos, Departamento de Estudios de Posgrado e Investigación, Universidad Nacional Autónoma de México
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Affiliation as printed
McMaster Immunology Research Centre, Schroeder Allergy and Immunology Research Institute, Department of Medicine, Faculty of Health Sciences, McMaster University
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ETH Zurich · Institute of Microbiology
Affiliation as printed
Institute of Microbiology, ETH Zurich
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National Institutes of Health · National Institute of Allergy and Infectious Diseases
Affiliation as printed
Center for Advanced Tissue Imaging Laboratory of Immune System Biology, NIAID, NIH
Lymphocyte Biology Section, Laboratory of Immune System Biology, NIAID, NIH
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National Institutes of Health · National Cancer Institute
Affiliation as printed
Surgical Oncology Program, National Cancer Institute, National Institutes of Health
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University of Cambridge · MRC Laboratory of Molecular Biology · Molecular Immunity Unit
Affiliation as printed
Cambridge Institute for Therapeutic Immunology and Infectious Diseases, University of Cambridge Department of Medicine, Molecular Immunity Unit, Laboratory of Molecular Biology
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National Institutes of Health · National Institute of Allergy and Infectious Diseases
Affiliation as printed
Lymphocyte Biology Section, Laboratory of Immune System Biology, NIAID, NIH
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National Institutes of Health · National Institute of Allergy and Infectious Diseases
Affiliation as printed
Lymphocyte Biology Section, Laboratory of Immune System Biology, NIAID, NIH
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National Institutes of Health · National Institute of Allergy and Infectious Diseases
Affiliation as printed
Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health
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National Institutes of Health · National Institute of Allergy and Infectious Diseases
Affiliation as printed
Lymphocyte Biology Section, Laboratory of Immune System Biology, NIAID, NIH
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Cleveland Clinic · Cleveland Clinic Lerner College of Medicine
Affiliation as printed
Florida Research and Innovation Center, Cleveland Clinic Lerner Research Institute
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Affiliation as printed
Institute of Anatomy, University of Zurich
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Wellcome/MRC Cambridge Stem Cell Institute
Affiliation as printed
Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre
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National Institutes of Health · National Institute of Allergy and Infectious Diseases
Affiliation as printed
Lymphocyte Biology Section, Laboratory of Immune System Biology, NIAID, NIH
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Affiliation as printed
Department of Medicine, McMaster University, Firestone Institute for Respiratory Health, St Joseph’s Healthcare
McMaster Immunology Research Centre, Schroeder Allergy and Immunology Research Institute, Department of Medicine, Faculty of Health Sciences, McMaster University
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National Institutes of Health · National Institute of Allergy and Infectious Diseases
Affiliation as printed
Laboratory of Clinical Immunology and Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health
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National Institutes of Health · National Heart, Lung, and Blood Institute
Affiliation as printed
Critical Care Medicine and Pulmonary Branch, National Heart, Lung and Blood Institute, National Institutes of Health
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ETH Zurich · Institute of Microbiology
Affiliation as printed
Institute of Microbiology, ETH Zurich
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National Institutes of Health · National Institute of Allergy and Infectious Diseases
Affiliation as printed
Center for Advanced Tissue Imaging Laboratory of Immune System Biology, NIAID, NIH
Lymphocyte Biology Section, Laboratory of Immune System Biology, NIAID, NIH
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National Institutes of Health · National Institute of Allergy and Infectious Diseases
Affiliation as printed
Center for Advanced Tissue Imaging Laboratory of Immune System Biology, NIAID, NIH
Lymphocyte Biology Section, Laboratory of Immune System Biology, NIAID, NIH
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