Multimeric, multivalent fusion carrier proteins for site-selective glycoconjugate vaccines simultaneously targeting Staphylococcus aureus and Pseudomonas aeruginosa

Chemical Science, vol. 16, pp. 5688–5700

Abstract

, respectively. A conjugation strategy based on selective targeting of a purposefully introduced histidine tag was developed to preserve the structure and antigenicity of epitopes from the two proteins, leveraging their dual role as a carrier and antigen. This multivalent, multimeric and multipathogen construct successfully elicited antibodies against all three antigens as well as functional protection. This proof-of-concept highlights the potential for advanced vaccines targeting polymicrobial infections and bacteria with complex pathogenesis calling for multivalent formulations. It also points out the power of site-selective conjugation as a tool for vaccine manufacturing.

Authors 12

1. Charlotte Sorieul Aachen

   Leiden University

   Affiliation as printed

   GSK Siena, Via Fiorentina, 1, 53100 Siena SI, Italy

   Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, Netherlands

2. Bartal Mikladal
   Affiliation as printed

   GSK Siena, Via Fiorentina, 1, 53100 Siena SI, Italy

3. Dung‐Yeh Wu Aachen

   Leiden University

   Affiliation as printed

   GSK Siena, Via Fiorentina, 1, 53100 Siena SI, Italy

   Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, Netherlands

4. Barbara Brogioni
   Affiliation as printed

   GSK Siena, Via Fiorentina, 1, 53100 Siena SI, Italy

5. Cinzia Giovani
   Affiliation as printed

   GSK Siena, Via Fiorentina, 1, 53100 Siena SI, Italy

6. Giusy Manuela Adamo
   Affiliation as printed

   GSK Siena, Via Fiorentina, 1, 53100 Siena SI, Italy

7. Giacomo Romagnoli
   Affiliation as printed

   GSK Siena, Via Fiorentina, 1, 53100 Siena SI, Italy

8. Immaculada Margarit
   Affiliation as printed

   GSK Siena, Via Fiorentina, 1, 53100 Siena SI, Italy

9. Jeroen D. C. Codée Aachen

   Leiden University

   Affiliation as printed

   Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden, Netherlands

10. Maria Rosaria Romano
    Affiliation as printed

    GSK Siena, Via Fiorentina, 1, 53100 Siena SI, Italy

11. Filippo Carboni corresponding
    Affiliation as printed

    GSK Siena, Via Fiorentina, 1, 53100 Siena SI, Italy

12. Roberto Adamo corresponding
    Affiliation as printed

    GSK Siena, Via Fiorentina, 1, 53100 Siena SI, Italy