Practical advice for extracellular vesicle isolation in plant–microbe interactions: Concerns, considerations, and conclusions

Journal of Extracellular Vesicles, vol. 13, pp. e70022

Abstract

In recent years, extracellular vesicles (EVs) have emerged as novel key players in plant-microbe interactions. While it is immensely useful to draw on the established "minimal information for studies of extracellular vesicles" (MISEV) guidelines and precedents in mammalian systems, working with plants and their associated microbes poses specific challenges. To navigate researchers through these obstacles, we offer detailed step-by-step suggestions for those embarking on EV research in the context of plant-microbe interactions. The advice is based on recent publications and our collective experience from the diverse plant and microbe systems studied in a dedicated research consortium. We provide considerations for experimental design, optimization, quality control, and recommendations on how to increase yield, purity, and reproducibility of EV isolation. With this perspective article, we aim not only to assist researchers in our field but also to promote discussions on plant and microbe EVs in the broader EV community.

Authors 12

1. Hannah Thieron Aachen Unit for Plant Molecular Cell Biology Institute for Biology I

   RWTH Aachen University

   Affiliation as printed

   Unit for Plant Molecular Cell Biology, Institute for Biology I RWTH Aachen University Aachen Germany

2. Laura Krassini

   Ludwig-Maximilians-Universität München

   Affiliation as printed

   LMU Munich Biocenter Ludwig‐Maximilian‐University of Munich Munich Germany

3. Seomun Kwon

   Cluster of Excellence on Plant Sciences · Heinrich Heine University Düsseldorf

   Affiliation as printed

   Institute for Microbiology, Cluster of Excellence on Plant Sciences Heinrich‐Heine University Düsseldorf Düsseldorf Germany

4. Sabrine Nasfi

   Justus-Liebig-Universität Gießen

   Affiliation as printed

   Institute of Phytopathology, Research Centre for BioSystems Land Use and Nutrition, Justus‐Liebig‐University Giessen Giessen Germany

5. Lorenz Oberkofler

   Ludwig-Maximilians-Universität München

   Affiliation as printed

   LMU Munich Biocenter Ludwig‐Maximilian‐University of Munich Munich Germany

6. Alessa Ruf

   Ludwig-Maximilians-Universität München

   Affiliation as printed

   LMU Munich Biocenter Ludwig‐Maximilian‐University of Munich Munich Germany

7. Julia Kehr

   Universität Hamburg

   Affiliation as printed

   Institute of Plant Science and Microbiology, Department of Biology Universität Hamburg Hamburg Germany

8. Karl‐Heinz Kogel

   Justus-Liebig-Universität Gießen

   Affiliation as printed

   Institute of Phytopathology, Research Centre for BioSystems Land Use and Nutrition, Justus‐Liebig‐University Giessen Giessen Germany

9. Arne Weiberg

   Ludwig-Maximilians-Universität München

   Affiliation as printed

   LMU Munich Biocenter Ludwig‐Maximilian‐University of Munich Munich Germany

10. Michael Feldbrügge

    Cluster of Excellence on Plant Sciences · Heinrich Heine University Düsseldorf

    Affiliation as printed

    Institute for Microbiology, Cluster of Excellence on Plant Sciences Heinrich‐Heine University Düsseldorf Düsseldorf Germany

11. Silke Robatzek

    Ludwig-Maximilians-Universität München

    Affiliation as printed

    LMU Munich Biocenter Ludwig‐Maximilian‐University of Munich Munich Germany

12. Ralph Panstruga corresponding Aachen Unit for Plant Molecular Cell Biology Institute for Biology I

    RWTH Aachen University

    Affiliation as printed

    Unit for Plant Molecular Cell Biology, Institute for Biology I RWTH Aachen University Aachen Germany