Fast spectroscopic and multisensor methods for analysis of glucosamine and hyaluronic acid in dietary supplements

Microchemical Journal, vol. 207, pp. 112116

Abstract

There is a lack of fast and inexpensive analytical methods for quantification of key ingredients in dietary supplements. Here we explore the potential of near infrared (NIR) spectrometry, attenuated total reflection infrared (ATR-IR) spectrometry and potentiometric multisensor system (MSS) in quantitative determination of glucosamine and hyaluronic acid in commercial samples of dietary supplements. All three methods have demonstrated their applicability for this task when combined with chemometric data processing. Principal Component Analysis (PCA) revealed similarities across the three techniques, indicating the presence of distinct sample composition. Partial least squares (PLS) models were constructed for glucosamine and hyaluronic acid quantification. The root mean square error of cross validation (RMSECV) for glucosamine quantification varied between 7.7 wt% and 8.9 wt%. NIR spectrometry has demonstrated the best accuracy for hyaluronic acid (RMSECV = 9.9 wt%), while ATR-IR and MSS yielded somewhat worse performance with RMSECV values of 12.1 and 11.3 wt%, respectively. The findings of this study indicated that NIR, ATR-IR and MSS exhibit reduced accuracy in comparison to complex and high-precision analytical techniques. However, they can be employed for the rapid, semi-quantitative evaluation of glucosamine and hyaluronic acid in dietary supplements, with the possibility of integration into routine quality control procedures.

Authors 5

1. Fabienne Lang Aachen Department of Chemistry and Biotechnology

   FH Aachen

   Affiliation as printed

   FH Aachen University of Applied Sciences, Department of Chemistry and Biotechnology, Heinrich-Mußmann-Straße 1-5, D-52428 Jülich, Germany

2. Klaudia Adels Aachen Department of Chemistry and Biotechnology

   FH Aachen

   Affiliation as printed

   FH Aachen University of Applied Sciences, Department of Chemistry and Biotechnology, Heinrich-Mußmann-Straße 1-5, D-52428 Jülich, Germany

3. Vitaly V. Panchuk

   St Petersburg University · Institute for Analytical Instrumentation

   Affiliation as printed

   Institute for Analytical Instrumentation RAS, Ul. Ivana Chernykh 31-33, 198095 St. Petersburg, Russia

   Institute of Chemistry, St Petersburg University, Universitetskaya nab. 7/9, 198034 St Petersburg, Russia

4. Dmitry O. Kirsanov

   St Petersburg University

   Affiliation as printed

   Institute of Chemistry, St Petersburg University, Universitetskaya nab. 7/9, 198034 St Petersburg, Russia

5. Yulia Borisovna Monakhova corresponding Aachen Department of Chemistry and Biotechnology

   Saratov State University · FH Aachen

   Affiliation as printed

   FH Aachen University of Applied Sciences, Department of Chemistry and Biotechnology, Heinrich-Mußmann-Straße 1-5, D-52428 Jülich, Germany

   Saratov State University, Institute of Chemistry, Astrakhanskaya Street 83, 410012 Saratov, Russia