T cell subset composition differs between blood and cerebrospinal fluid in amyotrophic lateral sclerosis

Clinical Immunology, vol. 265, pp. 110270

Abstract

Inflammation is a hallmark of amyotrophic lateral sclerosis (ALS) and is often assessed through biological samples. Due to the easier access, peripheral blood is more commonly phenotyped instead of cerebrospinal fluid (CSF) or affected tissues in ALS. Here, using flow cytometry, we compared the composition of T cell subsets in blood and CSF in ALS patients. We found consistent but weak correlations between blood and CSF for all T cell subsets examined. This finding implies that blood and CSF offer complementary information when characterizing T cell immunity in ALS and blood may not be used as a surrogate for CSF.

Authors 6

1. Solmaz Yazdani

   Karolinska Institutet

   Affiliation as printed

   Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden

2. Anikó Lovik Aachen Institute of Psychology, Leiden University

   Leiden University · Karolinska Institutet

   Affiliation as printed

   Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden; Institute of Psychology, Leiden University, Leiden, the Netherlands

   Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden

   Institute of Psychology, Leiden University, Leiden, the Netherlands

3. Christina Seitz

   Karolinska Institutet

   Affiliation as printed

   Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden

4. Caroline Ingre

   Karolinska University Hospital · Karolinska Institutet

   Affiliation as printed

   Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden; Department of Neurology, Karolinska University Hospital, Stockholm, Sweden

   Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden

   Department of Neurology, Karolinska University Hospital, Stockholm, Sweden

5. Fang Fang

   Karolinska Institutet

   Affiliation as printed

   Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden

6. John Andersson corresponding

   Karolinska Institutet

   Affiliation as printed

   Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden. Electronic address: john.andersson@ki.se

   Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden