3D trajectory data for "Physiological adaptation in flagellar architecture improves Vibrio alginolyticus chemotaxis in complex environments"

Harvard Dataverse

Abstract

3D trajectory data for "Physiological adaptation in flagellar architecture improves Vibrio alginolyticus chemotaxis in complex environments" in press at PNAS by Marianne Grognot, Jong Woo Nam, Lauren E. Elson & Katja M. Taute* *taute@rowland.harvard.edu An earlier version of the manuscript is available at https://www.biorxiv.org/content/10.1101/2023.02.06.526967v1 The .mat files provide the trajectory datasets 0 to 7 as defined in Tables S1 and S2 in the Supplementary Information, in a Matlab structure format. Trajectory position data are ADMM filtered as described in the Methods section of the manuscript. Each file contains multiple Matlab structures starting with "V_P" (for the Pof-only strain) or "V_PL" (for the WT strain), followed by dataset name (e.g., "1a") as defined in the SI Tables. Each structure V contains the following fields: * V.Speeds is a cell array where each cell corresponds to one trajectory. The trajectory is described by an array with the following columns: - column 1: frame number - column 2-4 : x, y and z positions (in micrometers) - column 6-8 : instantaneous speed in x, y and z (micrometers per second) - column 9 : absolute value of instantaneous speed (micrometers per second) - column 10 : local rate of angular change, as defined in Methods (degrees/frame) * V.Parameters is a simplified structure with descriptive parameters, containing: V.Parameters.fps : frame rate of the recording (frames per second) V.Parameters.medium : the medium filling the gradient channel of the chemotaxis chamber (Dataset 1-6) or the motility chamber (Dataset 7). (optional) V.Parameters.growth : indicates the growth medium used, if the strains were grown in another medium than MB + 7% PVP.

Authors 2

1. Marianne Grognot Aachen

   RWTH Aachen University

   Affiliation as printed

   RWTH Aachen University

   (RWTH Aachen University)

2. Katja M. Taute

   Harvard University

   Affiliation as printed

   Harvard University

   (Harvard University)