A systematic comparison of Kapton-based HARE chips for fixed-target serial crystallography
Cell Reports Physical Science, vol. 5, pp. 101987
Abstract
Fixed targets are a versatile sample delivery method for serial crystallography experiments and a favorable choice regarding sample efficiency. The hit-and-return (HARE) chips utilize pyramidal-shaped features to pre-position crystals in a regular pattern. Typically, these micron-sized features are chemically etched into single-crystal silicon wafers providing a rigid sample mount with minimal background. As polymer chips are emerging as an alternative, here we report the production of HARE chips identical to silicon with pyramidal-shaped features and micron accuracy by laser ablating Kapton foils. We systematically compare the serial crystallography experiments of five different proteins using both Kapton and silicon HARE chips. Comparing these 15 structures, Kapton HARE chips yield data quality that is comparable to the silicon chips. Therefore, we propose that Kapton is a viable and cost-effective alternative support material.
Authors 13
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University Medical Center Hamburg-Eppendorf
Affiliation as printed
University Medical Center Hamburg-Eppendorf (UKE), Martinistr. 52, 20246 Hamburg, Germany
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University Medical Center Hamburg-Eppendorf
Affiliation as printed
University Medical Center Hamburg-Eppendorf (UKE), Martinistr. 52, 20246 Hamburg, Germany
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European Molecular Biology Laboratory
Affiliation as printed
European Molecular Biology Laboratory (EMBL), Hamburg Unit, Notkestr. 85, 22761 Hamburg, Germany
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University Medical Center Hamburg-Eppendorf
Affiliation as printed
University Medical Center Hamburg-Eppendorf (UKE), Martinistr. 52, 20246 Hamburg, Germany
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University Medical Center Hamburg-Eppendorf
Affiliation as printed
University Medical Center Hamburg-Eppendorf (UKE), Martinistr. 52, 20246 Hamburg, Germany
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University Medical Center Hamburg-Eppendorf
Affiliation as printed
University Medical Center Hamburg-Eppendorf (UKE), Martinistr. 52, 20246 Hamburg, Germany
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European Molecular Biology Laboratory
Affiliation as printed
European Molecular Biology Laboratory (EMBL), Hamburg Unit, Notkestr. 85, 22761 Hamburg, Germany
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Universität Hamburg · Max Planck Institute for the Structure and Dynamics of Matter
Affiliation as printed
Institute for Nanostructure and Solid State Physics, Universität Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany
Max-Planck-Institute for Structure and Dynamics of Matter, Luruper Chaussee 149, 22761 Hamburg, Germany
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Gang Lü Aachen
Affiliation as printed
RWTH Aachen University, Templergraben 55, 52062 Aachen, Germany
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Bernhard Suer Aachen
Affiliation as printed
RWTH Aachen University, Templergraben 55, 52062 Aachen, Germany
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University Medical Center Hamburg-Eppendorf · European Molecular Biology Laboratory
Affiliation as printed
European Molecular Biology Laboratory (EMBL), Hamburg Unit, Notkestr. 85, 22761 Hamburg, Germany
University Medical Center Hamburg-Eppendorf (UKE), Martinistr. 52, 20246 Hamburg, Germany
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Fraunhofer Institute for Laser Technology
Affiliation as printed
Fraunhofer Institute for Laser Technology ILT, Steinbachstr. 15, 52074 Aachen, Germany
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Eike C. Schulz corresponding
Universität Hamburg · University Medical Center Hamburg-Eppendorf · Max Planck Institute for the Structure and Dynamics of Matter
Affiliation as printed
Institute for Nanostructure and Solid State Physics, Universität Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany
Max-Planck-Institute for Structure and Dynamics of Matter, Luruper Chaussee 149, 22761 Hamburg, Germany
University Medical Center Hamburg-Eppendorf (UKE), Martinistr. 52, 20246 Hamburg, Germany
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