Establishing Personalized Blood Protein Reference Ranges Using Noninvasive Microsampling and Targeted Proteomics: Implications for Antidoping Strategies
Journal of Proteome Research, vol. 23, pp. 1779–1787
Abstract
To prevent doping practices in sports, the World Anti-Doping Agency implemented the Athlete Biological Passport (ABP) program, monitoring biological variables over time to indirectly reveal the effects of doping rather than detect the doping substance or the method itself. In the context of this program, a highly multiplexed mass spectrometry-based proteomics assay for 319 peptides corresponding to 250 proteins was developed, including proteins associated with blood-doping practices. "Baseline" expression profiles of these potential biomarkers in capillary blood (dried blood spots (DBS)) were established using multiple reaction monitoring (MRM). Combining DBS microsampling with highly multiplexed MRM assays is the best-suited technology to enhance the effectiveness of the ABP program, as it represents a cost-effective and robust alternative analytical method with high specificity and selectivity of targets in the attomole range. DBS data were collected from 10 healthy athlete volunteers over a period of 140 days (28 time points per participant). These comprehensive findings provide a personalized targeted blood proteome "fingerprint" showcasing that the targeted proteome is unique to an individual and likely comparable to a DNA fingerprint. The results can serve as a baseline for future studies investigating doping-related perturbations.
Authors 9
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Jewish General Hospital · Lady Davis Institute for Medical Research
Affiliation as printed
Segal Cancer Proteomics Centre, Lady Davis Institute for Medical Research, Jewish General Hospital, Montréal, Quebec H3T 1E2, Canada
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Jewish General Hospital · Lady Davis Institute for Medical Research · McGill University
Affiliation as printed
Division of Experimental Medicine, McGill University, Montréal, Quebec H4A 3J1, Canada
Segal Cancer Proteomics Centre, Lady Davis Institute for Medical Research, Jewish General Hospital, Montréal, Quebec H3T 1E2, Canada
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Affiliation as printed
University of Victoria-Genome BC Proteomics Centre, Victoria, British Columbia V8Z 7X8, Canada
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Jewish General Hospital · Lady Davis Institute for Medical Research
Affiliation as printed
Segal Cancer Proteomics Centre, Lady Davis Institute for Medical Research, Jewish General Hospital, Montréal, Quebec H3T 1E2, Canada
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Leiden University Medical Center
Affiliation as printed
Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden 2333 ZC, The Netherlands
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Affiliation as printed
University of Victoria-Genome BC Proteomics Centre, Victoria, British Columbia V8Z 7X8, Canada
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CancerCare Manitoba · University of Manitoba
Affiliation as printed
CancerCare Manitoba Research Institute, Winnipeg, Manitoba R3E 0V9, Canada
Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba R3E 0J9, Canada
Department of Internal Medicine, University of Manitoba, Winnipeg, Manitoba R3E 3P4, Canada
Manitoba Centre for Proteomics and Systems Biology, Winnipeg, Manitoba R3E 3P4, Canada
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German Sport University Cologne
Affiliation as printed
European Monitoring Center for Emerging Doping Agents (EuMoCEDA), Cologne/Bonn 50933, Germany
Institute of Biochemistry, Center for Preventive Doping Research, German Sport University Cologne, Cologne 50933, Germany
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Christoph H. Borchers corresponding
Jewish General Hospital · Lady Davis Institute for Medical Research · McGill University
Affiliation as printed
Department of Pathology, McGill University, Montréal, Quebec H4A 3J1, Canada
Division of Experimental Medicine, McGill University, Montréal, Quebec H4A 3J1, Canada
Gerald Bronfman Department of Oncology, McGill University, Montréal, Quebec H4A 3T2, Canada
Segal Cancer Proteomics Centre, Lady Davis Institute for Medical Research, Jewish General Hospital, Montréal, Quebec H3T 1E2, Canada
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