Methyl-Sensitive Amplification Polymorphism (MSAP) Analysis Provides Insights into the DNA Methylation Underlying Heterosis in Kenaf (Hibiscus Cannabinus L.) Drought Tolerance

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Abstract

Kenaf (Hibiscus cannabinus L.) is an industrial fiber crop with significant heterosis and tolerance to abiotic stresses. However, the mechanism underlying heterosis in kenaf under drought conditions is completely unknown. In this study, F1 hybrids were remarkably tolerant to drought stress. Through methylation-sensitive amplification polymorphism (MSAP) analysis, we highlighted that total DNA methylation level under drought decreased by 11.2% in F1 and increased by 29.7% and 13.1% in parents CP079 and CP071, respectively. Unlike parents, the hypomethylation rate was 38% higher than hypermethylation in F1, the overall change in methylation pattern was higher in F1 (1.6%) than parents (0.4%). Furthermore, 35 key DNA fragments showing different methylation patterns were analyzed and found that the cytosine methylation status of DnaJ, ERF5, ZIP2, and PATL3 revealed changes in DNA methylation that may affect their respective expression level. Subsequently, virus-induced gene silencing (VIGS) mediated knockdown of the DnaJ, significantly increased the sensitivity of kenaf seedlings to drought. Overall, this study provides an epigenetic mechanism that could be used to improve the breeding strategy in kenaf.

Authors 15

1. Dengjie Luo
2. Shan Cao
3. Zengqiang Li

   Shihezi University

   Affiliation as printed

   Shihezi University

4. Meiqiong Tang
5. Caijin Wang
6. Enerand Mackon
7. Zhen Huang

   Shandong University · Nanjing University · Peking University · Chinese Academy of Medical Sciences & Peking Union Medical College · Peking University First Hospital · East China University of Science and Technology · Nanchang University · Army Medical University · Fujian Normal University

   Affiliation as printed

   Army Medical University

   Chinese Academy of Medical Sciences & Peking Union Medical College

   East China University of Science and Technology

   Fujian Normal University

   Nanchang University

   Nanjing University

   Peking University

   Peking University First Hospital

   Shandong University

8. Jiao Pan

   Hunan Agricultural University

   Affiliation as printed

   Hunan Agricultural University

9. Xia Wu

   Sichuan University · Zhejiang University · Peking Union Medical College Hospital · Hunan University · Shanghai First People's Hospital · Shaanxi University of Science and Technology · Heilongjiang Bayi Agricultural University

   Affiliation as printed

   Heilongjiang Bayi Agricultural University

   Hunan University

   Peking Union Medical College Hospital

   Shaanxi University of Science and Technology

   Shanghai First People's Hospital

   Sichuan University

   Zhejiang University

10. Qijing Wu
11. Hui Zhang

    Sun Yat-sen University · Johns Hopkins University · University College London · Chinese Academy of Sciences · Fudan University · Guangdong University of Technology · Northeast Agricultural University · Zhejiang University · Chinese Academy of Medical Sciences & Peking Union Medical College · Tianjin Medical University Cancer Institute and Hospital · Tianjin Medical University · Montefiore Medical Center · South China Agricultural University · Huazhong Agricultural University · Tongji University · Huashan Hospital · Yunnan Normal University · Jiangsu Academy of Agricultural Sciences · Shanghai University · Second Affiliated Hospital of Xi'an Jiaotong University · Peking University People's Hospital · Zhongda Hospital Southeast University · Guangzhou Medical University · The First Affiliated Hospital, Sun Yat-sen University · Harbin Medical University · Guangdong Provincial People's Hospital · Beijing Academy of Agriculture and Forestry Sciences · Yangzhou University · First Hospital of China Medical University · First Affiliated Hospital of GuangXi Medical University · Chinese Center For Disease Control and Prevention · Qingdao University · Affiliated Hospital of Qingdao University · Guizhou University · Xinjiang Medical University · First Affiliated Hospital of Xinjiang Medical University · First Hospital of Shanxi Medical University · Second Hospital of Shanxi Medical University · Jinzhou Medical University

    Affiliation as printed

    Affiliated Hospital of Qingdao University

    Beijing Academy of Agricultural and Forestry Sciences

    Chinese Academy of Medical Sciences & Peking Union Medical College

    Chinese Academy of Sciences

    Chinese Center For Disease Control and Prevention

    First Affiliated Hospital of GuangXi Medical University

    First Affiliated Hospital of Sun Yat-sen University

    First Affiliated Hospital of Xinjiang Medical University

    First Hospital of China Medical University

    First Hospital of Shanxi Medical University

    Fudan University

    Guangdong Provincial People's Hospital

    Guangdong University of Technology

    Guangzhou Medical University

    Guizhou University

    Harbin Medical University

    Huashan Hospital

    Huazhong Agricultural University

    Jiangsu Academy of Agricultural Sciences

    Johns Hopkins University

    Liaoning Medical University

    Montefiore Medical Center

    North West Agriculture and Forestry University

    Northeast Agricultural University

    Peking University People's Hospital

    Qingdao University

    Second Affiliated Hospital of Xi'an Jiaotong University

    Second Hospital of Shanxi Medical University

    Shanghai University

    South China Agricultural University

    Sun Yat-sen University

    Tianjin Medical University

    Tianjin Medical University Cancer Institute and Hospital

    Tongji University

    University College London

    Xinjiang Medical University

    Yangzhou University

    Yunnan Normal University

    Zhejiang University

    Zhongda Hospital Southeast University

12. Renxue Li
13. Xin Li Aachen

    RWTH Aachen University · Sun Yat-sen University · University of Alabama at Birmingham · University of Pennsylvania · Shanghai Jiao Tong University · Beijing Normal University · Shenyang Agricultural University · Nanjing University · Baylor College of Medicine · Fudan University · Sichuan University · Chinese Academy of Medical Sciences & Peking Union Medical College · Jilin University · Peking University First Hospital · Hubei University of Technology · Tongji University · XinHua Hospital · Central South University · City University of Hong Kong · University of South China · Southern Medical University · Nanjing Medical University · Jinan University · Army Medical University · Southwest Medical University · Harbin Medical University · Fu Wai Hospital · China Agricultural University · Tianjin Medical University General Hospital · Henan University · Chinese PLA General Hospital · Yangtze University · Taizhou University · Taizhou University · Guilin University of Technology · Qingdao University · Qinghai University · Qingdao Agricultural University · Shanghai University of Sport · Shaanxi Provincial People's Hospital · Kingmed Diagnostics · Tea Research Institute · Wuhan University of Science and Technology · Henan Medical University · South China Institute Of Environmental Sciences

    Affiliation as printed

    Army Medical University

    Baylor College of Medicine

    Beijing Normal University

    Central South University

    China Agricultural University

    Chinese Academy of Medical Sciences & Peking Union Medical College

    Chinese PLA General Hospital

    City University of Hong Kong

    Fudan University

    Fuwai Hospital

    Guilin University of Technology

    Harbin Medical University

    Henan University

    Hubei University of Technology

    Jilin University

    Jinan University

    Kingmed Diagnostics

    Nanjing Medical University

    Nanjing University

    Peking University First Hospital

    Qingdao Agricultural University

    Qingdao University

    Qinghai University

    RWTH Aachen University

    Shaanxi Provincial People's Hospital

    Shanghai Jiao Tong University

    Shanghai University of Sport

    Shenyang Agricultural University

    Sichuan University

    South China Institute Of Environmental Sciences

    Southern Medical University

    Southwest Medical University

    Sun Yat-sen University

    Taizhou University

    Tea Research Institute

    Tianjin Medical University General Hospital

    Tongji University

    University of Alabama at Birmingham

    University of Pennsylvania

    University of South China

    Wuhan University of Science and Technology

    XinHua Hospital

    Xinxiang Medical University

    Yangtze University

14. Ru Li

    Virginia Commonwealth University · Guangzhou Medical University

    Affiliation as printed

    Guangzhou Medical University

    Virginia Commonwealth University

15. Peng Chen

    Zhejiang University · University of Science and Technology of China · Hubei University of Technology · Hunan University · China Three Gorges University · Nanchang University · First Affiliated Hospital of Nanchang University · Affiliated Hospital of Guizhou Medical University · China Agricultural University · Shandong Academy of Agricultural Sciences · China Academy of Chinese Medical Sciences · Renmin Hospital of Wuhan University · Guizhou University · Third People's Hospital of Chengdu · First Affiliated Hospital of Guangzhou University of Chinese Medicine · Second Affiliated Hospital of Jilin University · Zhejiang Taizhou Hospital

    Affiliation as printed

    Affiliated Hospital of Guizhou Medical University

    Chengdu Third People's Hospital

    China Academy of Chinese Medical Sciences

    China Agricultural University

    China Three Gorges University

    First Affiliated Hospital of Guangzhou University of Chinese Medicine

    First Affiliated Hospital of Nanchang University

    Guizhou University

    Hubei University of Technology

    Hunan University

    Nanchang University

    Renmin Hospital of Wuhan University

    Second Affiliated Hospital of Jilin University

    Shandong Academy of Agricultural Sciences

    University of Science and Technology of China

    Zhejiang Taizhou Hospital

    Zhejiang University