An integrated approach towards extracting structural characteristics of chlorosomes from a bchQ mutant of Chlorobaculum tepidum

Physical Chemistry Chemical Physics, vol. 26, pp. 15856–15867

Abstract

The structure of the bchQ mutant of Cba. tepidum , with predominantly [8-Et, 12-Et]BChl c , is resolved using solid-state NMR, cryo-EM, and optical spectroscopy. Syn – anti parallel stacks run vertically with a subunit axial displacement of 1.49 nm.

Authors 11

1. Lolita Dsouza Aachen

   Leiden University

   Affiliation as printed

   Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2300 RA, Leiden, The Netherlands

2. Xinmeng Li Aachen

   Leiden University · University of Oslo

   Affiliation as printed

   Department of Chemistry and Hylleraas Centre for Quantum Molecular Sciences, University of Oslo, 0315, Oslo, Norway

   Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2300 RA, Leiden, The Netherlands

3. Vesna Erić

   University of Groningen · Zernike Institute for Advanced Materials

   Affiliation as printed

   Zernike Institute of Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG, The Netherlands

4. Thomas L. C. Jansen

   University of Groningen · Zernike Institute for Advanced Materials

   Affiliation as printed

   Zernike Institute of Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG, The Netherlands

5. Alfred R. Holzwarth

   Max Planck Institute for Chemical Energy Conversion

   Affiliation as printed

   Max Planck Institute for Chemical Energy Conversion, Stiftstraße 34-36, 45470, Mülheim an der Ruhr, Germany

6. Francesco Buda Aachen

   Leiden University

   Affiliation as printed

   Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2300 RA, Leiden, The Netherlands

7. Donald A. Bryant

   Pennsylvania State University

   Affiliation as printed

   Department for Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA

8. Salima Bahri

   Utrecht University

   Affiliation as printed

   NMR Spectroscopy, Bijvoet center for Biomolecular Research, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands

9. Karthick Babu Sai Sankar Gupta Aachen

   Leiden University

   Affiliation as printed

   Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2300 RA, Leiden, The Netherlands

10. G. J. A. Sevink corresponding Aachen

    Leiden University

    Affiliation as printed

    Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2300 RA, Leiden, The Netherlands

11. Huub J. M. de Groot corresponding Aachen

    Leiden University

    Affiliation as printed

    Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2300 RA, Leiden, The Netherlands