Identifying Differences in Molecular Characteristics Relevant for Remodeling of Periodontal Ligament Stem Cells from the Upper and Lower Jaw

International Journal of Molecular Sciences, vol. 25, pp. 3207

Abstract

Periodontal defects' localization affects wound healing and bone remodeling, with faster healing in the upper jaw compared to the lower jaw. While differences in blood supply, innervation, and odontogenesis contribute, cell-intrinsic variances may exist. Few studies explored cell signaling in periodontal ligament stem cells (PDLSC), overlooking mandible-maxilla disparitiesUsing kinomics technology, we investigated molecular variances in PDLSC. Characterization involved stem cell surface markers, proliferation, and differentiation capacities. Kinase activity was analyzed via multiplex kinase profiling, mapping differential activity in known gene regulatory networks. Upstream kinase analysis identified stronger EphA receptor expression in the mandible, potentially inhibiting osteogenic differentiation. The PI3K-Akt pathway showed higher activity in lower-jaw PDLSC. PDLSC from the upper jaw exhibit superior proliferation and differentiation capabilities. Differential activation of gene regulatory pathways in upper vs. lower-jaw PDLSC suggests implications for regenerative therapies.

Authors 10

1. Hanna Malyaran Aachen BioInterface Group Department of Orthodontics Interdisciplinary Center for Clinical Research (IZKF)

   Universitätsklinikum Aachen · RWTH Aachen University

   Affiliation as printed

   BioInterface Group, Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, 52056 Aachen, Germany

   Department of Orthodontics, University Hospital of RWTH Aachen, 52074 Aachen, Germany

   Interdisciplinary Center for Clinical Research (IZKF), RWTH Aachen University, 52074 Aachen, Germany

2. Rogerio Bastos Craveiro Aachen Department of Orthodontics

   Universitätsklinikum Aachen · RWTH Aachen University

   Affiliation as printed

   Department of Orthodontics, University Hospital of RWTH Aachen, 52074 Aachen, Germany

3. Sinan Mert

   LMU Klinikum · Ludwig-Maximilians-Universität München

   Affiliation as printed

   Division of Hand, Plastic and Aesthetic Surgery, LMU University Hospital Munich, 80336 Munich, Germany

4. Christian Niederau Aachen Department of Orthodontics

   Universitätsklinikum Aachen · RWTH Aachen University

   Affiliation as printed

   Department of Orthodontics, University Hospital of RWTH Aachen, 52074 Aachen, Germany

5. Sanne Lidewij Maas Aachen Institute for Molecular Cardiovascular Research (IMCAR) Interdisciplinary Center for Clinical Research (IZKF)

   RWTH Aachen University

   Affiliation as printed

   Aachen-Maastricht Institute for CardioRenal Disease (AMICARE), RWTH Aachen University, 52074 Aachen, Germany

   Institute for Molecular Cardiovascular Research (IMCAR), RWTH Aachen University, 52074 Aachen, Germany

   Interdisciplinary Center for Clinical Research (IZKF), RWTH Aachen University, 52074 Aachen, Germany

6. Emiel P. C. van der Vorst Aachen Institute for Molecular Cardiovascular Research (IMCAR) Interdisciplinary Center for Clinical Research (IZKF)

   Institut für Prophylaxe und Epidemiologie der Kreislaufkrankheiten · Ludwig-Maximilians-Universität München · RWTH Aachen University

   Affiliation as printed

   Aachen-Maastricht Institute for CardioRenal Disease (AMICARE), RWTH Aachen University, 52074 Aachen, Germany

   Institute for Cardiovascular Prevention (IPEK), Ludwig-Maximilians-University Munich, 80336 Munich, Germany

   Institute for Molecular Cardiovascular Research (IMCAR), RWTH Aachen University, 52074 Aachen, Germany

   Interdisciplinary Center for Clinical Research (IZKF), RWTH Aachen University, 52074 Aachen, Germany

7. Frank Hölzle Aachen Department of Oral and Maxillofacial Surgery

   Universitätsklinikum Aachen · RWTH Aachen University

   Affiliation as printed

   Department of Oral and Maxillofacial Surgery, University Hospital of RWTH Aachen, 52074 Aachen, Germany

8. Willi Jahnen‐Dechent Aachen BioInterface Group

   RWTH Aachen University

   Affiliation as printed

   BioInterface Group, Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, 52056 Aachen, Germany

9. Michael F. Wolf Aachen Department of Orthodontics

   Universitätsklinikum Aachen · RWTH Aachen University

   Affiliation as printed

   Department of Orthodontics, University Hospital of RWTH Aachen, 52074 Aachen, Germany

10. Sabine Neuß corresponding Aachen BioInterface Group Institute of Pathology

    RWTH Aachen University

    Affiliation as printed

    BioInterface Group, Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, 52056 Aachen, Germany

    Institute of Pathology, RWTH Aachen University, 52074 Aachen, Germany