Probing human immune cell mechanics using acoustic force spectroscopy

STAR Protocols, vol. 5, pp. 102861

Abstract

Immune cells continuously adapt their mechanical properties for proper circulation and elicitation of immune responses. Here, we provide a step-by-step protocol for probing the single-cell mechanical properties of primary human monocytes using acoustic force spectroscopy (AFS). We describe steps for the calibration of the AFS chips, the isolation of monocytes from buffy coats, and the probing of monocyte mechanics using AFS. We then detail the data analysis strategy. The protocol is useful for characterizing a wide range of immune cells under various conditions in physiology and pathology. For complete details on the use and execution of this protocol, please refer to Evers et al. 1 and Evers et al.2

Authors 3

1. Tom M. J. Evers corresponding Aachen Laboratory for Interdisciplinary Medical Innovations Centre for Interdisciplinary Genome Research Medical Systems Biophysics and Bioengineering Leiden Academic Centre for Drug Research Faculty of Mathematics and Natural Sciences

   Leiden University

   Affiliation as printed

   Medical Systems Biophysics and Bioengineering, Leiden Academic Centre for Drug Research, Faculty of Mathematics and Natural Sciences, Leiden University, Einsteinweg 55, 2333CC Leiden, the Netherlands; Laboratory for Interdisciplinary Medical Innovations, Centre for Interdisciplinary Genome Research, Leiden University, Einsteinweg 55, 2333CC Leiden, the Netherlands. Electronic address: t.m.j.evers@lacdr.leidenuniv.nl

   Laboratory for Interdisciplinary Medical Innovations, Centre for Interdisciplinary Genome Research, Leiden University, Einsteinweg 55, 2333CC Leiden, the Netherlands

   Medical Systems Biophysics and Bioengineering, Leiden Academic Centre for Drug Research, Faculty of Mathematics and Natural Sciences, Leiden University, Einsteinweg 55, 2333CC Leiden, the Netherlands

2. Mehrad Babaei Aachen Laboratory for Interdisciplinary Medical Innovations Centre for Interdisciplinary Genome Research Medical Systems Biophysics and Bioengineering Leiden Academic Centre for Drug Research Faculty of Mathematics and Natural Sciences

   Leiden University

   Affiliation as printed

   Medical Systems Biophysics and Bioengineering, Leiden Academic Centre for Drug Research, Faculty of Mathematics and Natural Sciences, Leiden University, Einsteinweg 55, 2333CC Leiden, the Netherlands; Laboratory for Interdisciplinary Medical Innovations, Centre for Interdisciplinary Genome Research, Leiden University, Einsteinweg 55, 2333CC Leiden, the Netherlands

   Laboratory for Interdisciplinary Medical Innovations, Centre for Interdisciplinary Genome Research, Leiden University, Einsteinweg 55, 2333CC Leiden, the Netherlands

   Medical Systems Biophysics and Bioengineering, Leiden Academic Centre for Drug Research, Faculty of Mathematics and Natural Sciences, Leiden University, Einsteinweg 55, 2333CC Leiden, the Netherlands

3. Alireza Mashaghi corresponding Aachen Laboratory for Interdisciplinary Medical Innovations Centre for Interdisciplinary Genome Research Medical Systems Biophysics and Bioengineering Leiden Academic Centre for Drug Research Faculty of Mathematics and Natural Sciences

   Leiden University

   Affiliation as printed

   Medical Systems Biophysics and Bioengineering, Leiden Academic Centre for Drug Research, Faculty of Mathematics and Natural Sciences, Leiden University, Einsteinweg 55, 2333CC Leiden, the Netherlands; Laboratory for Interdisciplinary Medical Innovations, Centre for Interdisciplinary Genome Research, Leiden University, Einsteinweg 55, 2333CC Leiden, the Netherlands. Electronic address: a.mashaghi.tabari@lacdr.leidenuniv.nl

   Laboratory for Interdisciplinary Medical Innovations, Centre for Interdisciplinary Genome Research, Leiden University, Einsteinweg 55, 2333CC Leiden, the Netherlands

   Medical Systems Biophysics and Bioengineering, Leiden Academic Centre for Drug Research, Faculty of Mathematics and Natural Sciences, Leiden University, Einsteinweg 55, 2333CC Leiden, the Netherlands