Prebiotic Cellulose–Pullulan Matrix as a “Vehicle” for Probiotic Biofilm Delivery to the Host Large Intestine

Polymers, vol. 16, pp. 30

Abstract

GG (LGG) bacteria in biofilm form. The new composition allows for delivering the bacteria to the digestive tract in a manner that improves their robustness compared with planktonic cells and released biofilm cells. Granules consisting of a polysaccharide matrix with probiotic biofilms (PMPB) with high cell density (>9 log CFU/g) were obtained by immobilization in the optimized nutrient medium. Successful probiotic loading was confirmed by fluorescence microscopy and scanning electron microscopy. The developed prebiotic polysaccharide matrix significantly enhanced LGG viability under acidic (pH 2.0) and bile salt (0.3%) stress conditions. Enzymatic extract of feces, mimicking colon fluid in terms of cellulase activity, was used to evaluate the intestinal release of probiotics. PMPB granules showed the ability to gradually release a large number of viable LGG cells in the model colon fluid. In vivo, the oral administration of PMPB granules in rats resulted in the successful release of probiotics in the colon environment. The biofilm-forming incubation method of immobilization on a complex polysaccharide matrix tested in this study has shown high efficacy and promising potential for the development of innovative biotechnologies.

Authors 8

1. Irina Savitskaya

   Al-Farabi Kazakh National University

   Affiliation as printed

   Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan

2. Sirina Zhantlessova corresponding

   Al-Farabi Kazakh National University

   Affiliation as printed

   Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan

3. Aida Kistaubayeva corresponding

   Al-Farabi Kazakh National University

   Affiliation as printed

   Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan

4. Ludmila V. Ignatova

   Al-Farabi Kazakh National University

   Affiliation as printed

   Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan

5. Dina Shokatayeva

   Al-Farabi Kazakh National University

   Affiliation as printed

   Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan

6. Yuriy A. Sinyavskiy
   Affiliation as printed

   Kazakh Academy of Nutrition, Almaty 050008, Kazakhstan

7. Almagul R. Kushugulova

   Nazarbayev University

   Affiliation as printed

   Laboratory of Human Microbiome and Longevity, Center for Life Sciences, National Laboratory Astana, Nazarbayev University, 53 Kabanbay Batyr Avenue, Astana 010000, Kazakhstan

8. Ilya Digel Aachen Institute for Bioengineering

   FH Aachen

   Affiliation as printed

   Institute for Bioengineering, Aachen University of Applied Sciences, Heinrich-Mußmann-Straße 1, D-52428 Jülich, Germany