3D-printed scaffolds with perfusable channels for low-cost large construct 3D cell culture

Current Directions in Biomedical Engineering, vol. 9, pp. 662–665

Abstract

Abstract This study presents a novel approach for threedimensional (3D) cell culture using a two-component system consisting of a 3D-printed scaffold structure combined with a perfusion channel. A polymeric scaffold structure with an overall size of 9 mm x 9 mm x 1 mm composed of a cubic lattice with a web thickness of 200 μm and pore size of 600 μm was 3D-printed using a hot UV-stereolithography (SLA) system. The perfusion channel with an inner diameter of 800 μm and channel wall pores of 300 μm for cell culture medium supply was 3D-printed with the same system and material. Scaffolds were investigated with respect to the printing accuracy by digital microscopy. Cytotoxicity of the materials was assessed using MTT-assay and Live/Dead staining. Scaffold were subsequently seeded with 3T3- fibroblasts within a fibrin-based hydrogel and then conditioned either statically or under passive perfusion using a hydrostatic pressure driven flow system. The results show that the scaffold structure and perfusion channel can be produced with high accuracy and stability allowing a supply of nutrient and oxygen via perfusion channel to the cells within the scaffold. This approach has potential for nutrient supply within larger constructs for tissue engineering and regenerative medicine applications.

Authors 4

1. Klaus Kreuels corresponding Aachen Chair for Laser Technology LLT

   RWTH Aachen University

   Affiliation as printed

   Chair for Laser Technology LLT, RWTH Aachen University, Steinbachstr. 15 , Aachen , Germany

2. Carina Schemmer Aachen Chair for Laser Technology LLT

   RWTH Aachen University

   Affiliation as printed

   Chair for Laser Technology LLT, RWTH Aachen University , Aachen , Germany

3. Maximilian Frederick Flesch Aachen Fraunhofer Institute for Laser Technology ILT

   Fraunhofer Institute for Laser Technology

   Affiliation as printed

   Fraunhofer Institute for Laser Technology ILT , Aachen , Germany

4. Arnold Gillner Aachen Chair for Laser Technology LLT Fraunhofer Institute for Laser Technology ILT

   Fraunhofer Institute for Laser Technology · RWTH Aachen University

   Affiliation as printed

   Chair for Laser Technology LLT, RWTH Aachen University , Aachen , Germany

   Fraunhofer Institute for Laser Technology ILT , Aachen , Germany