Discovering Matrix Adducts for Enhanced Metabolite Profiling with Stable Isotope-Labeled MALDI-MSI
bioRxiv (Cold Spring Harbor Laboratory)
Abstract
Abstract Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) is a widely used technique for spatial metabolomics analysis, but the matrix introduces spectral interferences that impede data processing. In this study, we present an experimental and computational workflow utilizing isotopic labeling to discover and annotate matrix adducts in MALDI-MSI. Our approach enables the removal of matrix-related signals, improving metabolite annotation accuracy, extending metabolome coverage, and facilitating the interpretation of tissue morphology.
Authors 9
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Gerard Baquer corresponding
Affiliation as printed
Department of Electronic Engineering, University Rovira i Virgili, Tarragona, Spain
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Affiliation as printed
Department of Analytical Chemistry and Organic Chemistry, Universitat Rovira i Virgili, Tarragona, Spain
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Affiliation as printed
Department of Electronic Engineering, University Rovira i Virgili, Tarragona, Spain
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Leiden University Medical Center
Affiliation as printed
Center of Proteomics and Metabolomics, Leiden University Medical Center, Leiden, the Netherlands
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María García‐Altares corresponding
Affiliation as printed
Department of Electronic Engineering, University Rovira i Virgili, Tarragona, Spain
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Bram Heijs Aachen Center of Proteomics and Metabolomics Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW)
Leiden University Medical Center · Novo Nordisk Foundation Center for Stem Cell Medicine
Affiliation as printed
Center of Proteomics and Metabolomics, Leiden University Medical Center, Leiden, the Netherlands
The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center, the Netherlands
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Affiliation as printed
Department of Analytical Chemistry and Organic Chemistry, Universitat Rovira i Virgili, Tarragona, Spain
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Centro de Investigación Biomédica en Red Diabetes y Enfermedades Metabólicas Asociadas · Institut de Recerca Biomèdica Catalunya Sud · Universitat Rovira i Virgili
Affiliation as printed
Department of Electronic Engineering, University Rovira i Virgili, Tarragona, Spain
Institut D’Investigacio Sanitaria Pere Virgili (IISPV), Tarragona, Spain
Spanish Biomedical Research Center in Diabetes and Associated Metabolic Disorders (CIBERDEM), Madrid, Spain
Institut D'Investigacio Sanitaria Pere Virgili (IISPV), Tarragona, Spain
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Centro de Investigación Biomédica en Red Diabetes y Enfermedades Metabólicas Asociadas · Institut de Recerca Biomèdica Catalunya Sud · Universitat Rovira i Virgili
Affiliation as printed
Department of Electronic Engineering, University Rovira i Virgili, Tarragona, Spain
Institut D’Investigacio Sanitaria Pere Virgili (IISPV), Tarragona, Spain
Spanish Biomedical Research Center in Diabetes and Associated Metabolic Disorders (CIBERDEM), Madrid, Spain
Institut D'Investigacio Sanitaria Pere Virgili (IISPV), Tarragona, Spain
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