A Flexible and Efficient Microfluidics Platform for the Characterization and Isolation of Novel Bacteriophages
Applied and Environmental Microbiology, vol. 89, pp. e0159622
Abstract
Bacteriophages are gaining increased attention for their potential application as agents to combat antibiotic-resistant infections. However, isolation and characterization of new phages are time consuming and limited by currently used methods. The microfluidics platform presented here allows the isolation and long-term analysis of phages and their effect on host cells with fluorescent light microscopy imaging. Furthermore, this new workflow allows high-throughput characterization of environmental samples for the identification of phages alongside gaining detailed insight into the host response. Taken together, this microfluidics platform will be a valuable tool for phage research, enabling faster and more efficient screening and characterization of host-phage interactions.
Authors 5
-
Adam Sidi Mabrouk Aachen Centre for Microbial Cell Biology Department of Microbial Sciences Institute of Biology, Leiden University
Affiliation as printed
Centre for Microbial Cell Biology, Leiden University, Leiden, The Netherlands
Department of Microbial Sciences, Institute of Biology, Leiden University, Leiden, The Netherlands
-
Véronique Ongenae Aachen Centre for Microbial Cell Biology Department of Microbial Sciences Institute of Biology, Leiden University
Affiliation as printed
Centre for Microbial Cell Biology, Leiden University, Leiden, The Netherlands
Department of Microbial Sciences, Institute of Biology, Leiden University, Leiden, The Netherlands
-
Dennis Claessen Aachen Centre for Microbial Cell Biology Department of Microbial Sciences Institute of Biology, Leiden University
Affiliation as printed
Centre for Microbial Cell Biology, Leiden University, Leiden, The Netherlands
Department of Microbial Sciences, Institute of Biology, Leiden University, Leiden, The Netherlands
-
Affiliation as printed
Department of Microbiology, University of Würzburg, Biocenter, Würzburg, Germany
-
Ariane Briegel corresponding Aachen Centre for Microbial Cell Biology Department of Microbial Sciences Institute of Biology, Leiden University
Affiliation as printed
Centre for Microbial Cell Biology, Leiden University, Leiden, The Netherlands
Department of Microbial Sciences, Institute of Biology, Leiden University, Leiden, The Netherlands
Cited by 13 stored of 13
13 results
No patents citing this paper on Lens.org (checked 2026-10-11).