Mouse Oocyte Characterization by Electrical Impedance Spectroscopy

Proceedings of IEEE Sensors/Proceedings of IEEE Sensors ..., pp. 1–4

Abstract

Artificial fertilization depends on the oocyte quality, especially on the zona pellucida. This gelatinous outer layer becomes soft before and hardens after sperm penetration. Here, we propose a setup that characterizes the stiffness of the zona pellucida of mouse oocytes by electrical impedance spectroscopy. Single oocytes are hydrodynamically trapped at an aperture, which is located between two ring-shaped electrodes. By applying weak negative pressures to the cell trap, the electrical impedance correlates with the stiffness of the zona pellucida.

Authors 4

1. Yuan Cao Aachen Institute of Materials in Electrical Engineering 1

   RWTH Aachen University

   Affiliation as printed

   Institute of Materials in Electrical Engineering 1, RWTH Aachen University,Aachen,Germany

   Institute of Materials in Electrical Engineering 1, RWTH Aachen University, Aachen, Germany

2. Julia Floehr Aachen

   Universitätsklinikum Aachen · RWTH Aachen University

   Affiliation as printed

   Helmholtz-Institute for Biomedical Engineering, Uniklinik RWTH Aachen University,Aachen,Germany

   Helmholtz-Institute for Biomedical Engineering, Uniklinik RWTH Aachen University, Aachen, Germany

3. Danyil Azarkh Aachen Institute of Materials in Electrical Engineering 1

   RWTH Aachen University

   Affiliation as printed

   Institute of Materials in Electrical Engineering 1, RWTH Aachen University,Aachen,Germany

   Institute of Materials in Electrical Engineering 1, RWTH Aachen University, Aachen, Germany

4. Uwe Schnakenberg Aachen Institute of Materials in Electrical Engineering 1

   RWTH Aachen University

   Affiliation as printed

   Institute of Materials in Electrical Engineering 1, RWTH Aachen University,Aachen,Germany

   Institute of Materials in Electrical Engineering 1, RWTH Aachen University, Aachen, Germany