Quantification of bowel ischaemia using real-time multispectral Single Snapshot Imaging of Optical Properties (SSOP)

Surgical Endoscopy, vol. 37, pp. 2395–2403

Abstract

BACKGROUND: Single snapshot imaging of optical properties (SSOP) is a relatively new non-invasive, real-time, contrast-free optical imaging technology, which allows for the real-time quantitative assessment of physiological properties, including tissue oxygenation (StO2). This study evaluates the accuracy of multispectral SSOP in quantifying bowel ischaemia in a preclinical experimental model. METHODS: In six pigs, an ischaemic bowel segment was created by dividing the arcade branches. Five regions of interest (ROIs) were identified on the bowel loop, as follows: ROI 1: central ischaemic; ROI 2: left marginal; ROI 3: left vascularised; ROI 4: right marginal; and ROI 5: right vascularised. The Trident imaging system, specifically developed for real-time tissue oxygenation imaging using SSOP, was used to image before (T0) and after ischaemia induction. Capillary and systemic lactates were measured at each time point (T0, T15, T30, T45, T60), as well as StO2 values acquired by means of SSOP (SSOP-StO2). RESULTS: The mean value of SSOP-StO2 in ROI 1 was 30.08 ± 6.963 and was significantly lower when compared to marginal ROIs (ROI 2 + ROI 4: 45.67 ± 10.02 p = < 0.0001), and to vascularised ROIs (ROI 3 + ROI 5: 48.08 ± 7.083 p = < 0.0001). SSOP-StO2 was significantly correlated with normalised lactates r = - 0.5892 p < 0.0001 and with histology r =- 0.6251 p = 0.0002. CONCLUSION: Multispectral SSOP allows for a contrast-free accurate assessment of small bowel perfusion identifying physiological tissue oxygenation as confirmed with perfusion biomarkers.

Authors 12

1. María Rita Rodríguez Luna corresponding

   Institut de Recherche contre les Cancers de l’Appareil Digestif · Université de Strasbourg

   Affiliation as printed

   Research Institute Against Digestive Cancer (IRCAD), 1, place de l'Hôpital, 67000, Strasbourg Cedex, France. rita.rodriguez-luna@ircad.fr

   University of Strasbourg, ICube Laboratory, Strasbourg, France. rita.rodriguez-luna@ircad.fr

   Research Institute Against Digestive Cancer (IRCAD), 1, place de l'Hôpital, 67000, Strasbourg Cedex, France

2. Nariaki Okamoto

   Institut de Recherche contre les Cancers de l’Appareil Digestif · Université de Strasbourg

   Affiliation as printed

   Research Institute Against Digestive Cancer (IRCAD), 1, place de l'Hôpital, 67000, Strasbourg Cedex, France

   University of Strasbourg, ICube Laboratory, Strasbourg, France

3. Lorenzo Cinelli

   Institut de Recherche contre les Cancers de l’Appareil Digestif · IRCCS Ospedale San Raffaele

   Affiliation as printed

   Department of Gastrointestinal Surgery, San Raffaele Hospital IRCCS, Milan, Italy

   Research Institute Against Digestive Cancer (IRCAD), 1, place de l'Hôpital, 67000, Strasbourg Cedex, France

4. Luca Baratelli

   Intuitive Surgical (Switzerland)

   Affiliation as printed

   Intuitive Surgical Sàrl, Aubonne, Switzerland

5. Silvère Ségaud

   Université de Strasbourg

   Affiliation as printed

   University of Strasbourg, ICube Laboratory, Strasbourg, France

6. Adriana Rodríguez-Gómez
   Affiliation as printed

   Department of Pathology, San Angel Inn Sur Hospital, Mexico City, Mexico

7. Deborah Susan Keller

   Main Line Health · Lankenau Medical Center

   Affiliation as printed

   Marks Colorectal Surgical Associates, Lankenau Medical Center, Main Line Health, Wynnewood, PA, USA

8. Elham Zonoobi Aachen Department of Surgery, Leiden University Medical Center

   Leiden University Medical Center

   Affiliation as printed

   Department of Surgery, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands

   Edinburgh Molecular Imaging Ltd. (EMI), Edinburgh, EH16 4UX, UK

9. Elisa Bannone

   University of Verona · Institut de Recherche contre les Cancers de l’Appareil Digestif

   Affiliation as printed

   Department of General and Pancreatic surgery - The Pancreas Institute, University of Verona, Verona, Italy

   Research Institute Against Digestive Cancer (IRCAD), 1, place de l'Hôpital, 67000, Strasbourg Cedex, France

10. Jacques Marescaux

    Institut de Recherche contre les Cancers de l’Appareil Digestif

    Affiliation as printed

    Research Institute Against Digestive Cancer (IRCAD), 1, place de l'Hôpital, 67000, Strasbourg Cedex, France

11. Michèle Diana

    Institut de Recherche contre les Cancers de l’Appareil Digestif · Université de Strasbourg

    Affiliation as printed

    Research Institute Against Digestive Cancer (IRCAD), 1, place de l'Hôpital, 67000, Strasbourg Cedex, France

    University of Strasbourg, ICube Laboratory, Strasbourg, France

12. Sylvain Gioux

    Intuitive Surgical (Switzerland) · Université de Strasbourg

    Affiliation as printed

    Intuitive Surgical Sàrl, Aubonne, Switzerland

    University of Strasbourg, ICube Laboratory, Strasbourg, France