A flexible and efficient microfluidics platform for the characterization and isolation of novel bacteriophages

bioRxiv (Cold Spring Harbor Laboratory)

Abstract

ABSTRACT Bacteriophages are viruses that infect bacteria. This property makes them highly suitable for varied uses in industry or in the development of the treatment of bacterial infections. However, the conventional methods that are used to isolate and analyze these bacteriophages from the environment are generally cumbersome and time-consuming. Here, we adapted a high-throughput microfluidic setup for long-term analysis of bacteriophage-bacteria interaction and demonstrate isolation of phages from environmental samples.

Authors 5

1. Adam Sidi Mabrouk Aachen Centre for Microbial Cell Biology Department of Microbial Sciences Institute of Biology, Leiden University

   Leiden University

   Affiliation as printed

   Centre for Microbial Cell Biology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands

   Department of Microbial Sciences, Institute of Biology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands

   Centre for Microbial Cell Biology, Leiden University, Sylviusweg 7, 333 BE Leiden, The Netherlands

2. Véronique Ongenae Aachen Centre for Microbial Cell Biology Department of Microbial Sciences Institute of Biology, Leiden University

   Leiden University

   Affiliation as printed

   Centre for Microbial Cell Biology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands

   Department of Microbial Sciences, Institute of Biology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands

   Centre for Microbial Cell Biology, Leiden University, Sylviusweg 7, 333 BE Leiden, The Netherlands

3. Dennis Claessen Aachen Centre for Microbial Cell Biology Department of Microbial Sciences Institute of Biology, Leiden University

   Leiden University

   Affiliation as printed

   Centre for Microbial Cell Biology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands

   Department of Microbial Sciences, Institute of Biology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands

   Centre for Microbial Cell Biology, Leiden University, Sylviusweg 7, 333 BE Leiden, The Netherlands

4. Susanne Brenzinger

   University of Würzburg

   Affiliation as printed

   Department of Microbiology, University of Würzburg, Biocenter, Am Hubland, Würzburg 97074, Germany

5. Ariane Briegel corresponding Aachen Centre for Microbial Cell Biology Department of Microbial Sciences Institute of Biology, Leiden University

   Leiden University

   Affiliation as printed

   Centre for Microbial Cell Biology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands

   Department of Microbial Sciences, Institute of Biology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands

   Centre for Microbial Cell Biology, Leiden University, Sylviusweg 7, 333 BE Leiden, The Netherlands