Molecular Quantity Variations in Human-Mandibular-Bone Osteoid

Calcified Tissue International, vol. 111, pp. 547–558

Abstract

Osteoid is a layer of new-formed bone that is deposited on the bone border during the process of new bone formation. This deposition process is crucial for bone tissue, and flaws in it can lead to bone diseases. Certain bone diseases, i.e. medication related osteonecrosis, are overexpressed in mandibular bone. Because mandibular bone presents different properties than other bone types, the data concerning osteoid formation in other bones are inapplicable for human-mandibular bone. Previously, the molecular distribution of other bone types has been presented using Fourier-transform infrared (FTIR) spectroscopy. However, the spatial distribution of molecular components of healthy-human-mandibular-bone osteoid in relation to histologic landmarks has not been previously presented and needs to be studied in order to understand diseases that occur human-mandibular bone. This study presents for the first time the variation in molecular distribution inside healthy-human-mandibular-bone osteoid by juxtaposing FTIR data with its corresponding histologic image obtained by autofluorescence imaging of its same bone section. During new bone formation, bone-forming cells produce an osteoid constituted primarily of type I collagen. It was observed that in mandibular bone, the collagen type I increases from the osteoblast line with the distance from the osteoblasts, indicating progressive accumulation of collagen during osteoid formation. Only later inside the collagen matrix, the osteoid starts to mineralize. When the mineralization starts, the collagen accumulation diminishes whereas the collagen maturation still continues. This chemical-apposition process in healthy mandibular bone will be used in future as a reference to understand different pathologic conditions that occur in human-mandibular bone.

Authors 8

1. Anni Palander corresponding

   University of Eastern Finland

   Affiliation as printed

   Institute of Dentistry, University of Eastern Finland, Yliopistonranta 1, Kuopio, 70210, Finland. anni.palander@tuni.fi

   Institute of Dentistry, University of Eastern Finland, Yliopistonranta 1, Kuopio, 70210, Finland

2. Laure Fauch

   University of Eastern Finland

   Affiliation as printed

   SIB Labs, University of Eastern Finland, Yliopistonranta 1, Kuopio, 70210, Finland

3. Mikael J. Turunen

   University of Eastern Finland

   Affiliation as printed

   Department of Applied Physics, University of Eastern Finland, Yliopistonranta 1, Kuopio, 70210, Finland

4. Hannah Dekker

   Academic Center for Dentistry Amsterdam · Amsterdam University Medical Centers · Vrije Universiteit Amsterdam

   Affiliation as printed

   Department of Oral and Maxillofacial Surgery/Oral Pathology, Amsterdam UMC and Academic Centre for Dentistry Amsterdam (ACTA), Vrije Universiteit Amsterdam, De Boelelaan, Amsterdam, 1117, The Netherlands

5. Engelbert A. J. M. Schulten

   Academic Center for Dentistry Amsterdam · Amsterdam University Medical Centers · Vrije Universiteit Amsterdam

   Affiliation as printed

   Department of Oral and Maxillofacial Surgery/Oral Pathology, Amsterdam UMC and Academic Centre for Dentistry Amsterdam (ACTA), Vrije Universiteit Amsterdam, De Boelelaan, Amsterdam, 1117, The Netherlands

6. Arto P. Koistinen

   University of Eastern Finland

   Affiliation as printed

   SIB Labs, University of Eastern Finland, Yliopistonranta 1, Kuopio, 70210, Finland

7. Nathalie Bravenboer Aachen Division of Endocrinology & Center for Bone Quality Department of Internal Medicine

   Leiden University · Leiden University Medical Center · Amsterdam University Medical Centers · Vrije Universiteit Amsterdam

   Affiliation as printed

   Department of Clinical Chemistry, Amsterdam UMC, Vrije Universiteit Amsterdam, De Boelelaan, Amsterdam, 1117, The Netherlands

   Division of Endocrinology and Center for Bone Quality, Department of Internal Medicine, Leiden University Medical Center, PO Box 9500, Leiden, The Netherlands

8. Arja M. Kullaa

   University of Eastern Finland

   Affiliation as printed

   Institute of Dentistry, University of Eastern Finland, Yliopistonranta 1, Kuopio, 70210, Finland

   SIB Labs, University of Eastern Finland, Yliopistonranta 1, Kuopio, 70210, Finland