Standardized hypoxia-reoxygenation protocol to assess posthypoxic neurobehavioral impairments and molecular mechanisms in Drosophila melanogaster

STAR Protocols, vol. 3, pp. 101634

Abstract

Hypoxia plays a pivotal role in the pathogenesis of major causes of mortality such as cerebral ischemia. Here, we present a standardized protocol for the induction of global hypoxia and reoxygenation in Drosophila melanogaster, with details on subsequent analysis of mortality, neurobehavioral impairments, and molecular mechanisms. This protocol emphasizes the importance of controlling and monitoring specific environmental parameters to ensure reproducible results. It also highlights profound differences that can arise from variations in the age and genotype of the flies. For complete details on the use and execution of this protocol, please refer to Habib et al. (2021).

Authors 4

1. Jennifer Jung Aachen Department of Neurology, Medical Faculty

   RWTH Aachen University

   Affiliation as printed

   Department of Neurology, Medical Faculty, RWTH Aachen University, 52074 Aachen, Germany

2. Aaron T. Fehr Aachen Department of Neurology, Medical Faculty

   RWTH Aachen University

   Affiliation as printed

   Department of Neurology, Medical Faculty, RWTH Aachen University, 52074 Aachen, Germany

3. Aaron Voigt Aachen Department of Neurology, Medical Faculty JARA Brain Institute Molecular Neuroscience and Neuroimaging (INM-11)

   Forschungszentrum Jülich · Jülich Aachen Research Alliance · RWTH Aachen University

   Affiliation as printed

   Department of Neurology, Medical Faculty, RWTH Aachen University, 52074 Aachen, Germany; JARA BRAIN Institute Molecular Neuroscience and Neuroimaging, Forschungszentrum Jülich GmbH and RWTH Aachen University, 52074 Aachen, Germany

   Department of Neurology, Medical Faculty, RWTH Aachen University, 52074 Aachen, Germany

   JARA BRAIN Institute Molecular Neuroscience and Neuroimaging, Forschungszentrum Jülich GmbH and RWTH Aachen University, 52074 Aachen, Germany

4. Pardes Habib corresponding Aachen Department of Neurology, Medical Faculty JARA Brain Institute Molecular Neuroscience and Neuroimaging (INM-11)

   Forschungszentrum Jülich · Jülich Aachen Research Alliance · RWTH Aachen University

   Affiliation as printed

   Department of Neurology, Medical Faculty, RWTH Aachen University, 52074 Aachen, Germany; JARA BRAIN Institute Molecular Neuroscience and Neuroimaging, Forschungszentrum Jülich GmbH and RWTH Aachen University, 52074 Aachen, Germany. Electronic address: phabib@ukaachen.de

   Department of Neurology, Medical Faculty, RWTH Aachen University, 52074 Aachen, Germany

   JARA BRAIN Institute Molecular Neuroscience and Neuroimaging, Forschungszentrum Jülich GmbH and RWTH Aachen University, 52074 Aachen, Germany