Multi-color flow cytometer with PIC-based structured illumination

Biomedical Optics Express, vol. 13, pp. 5098

Abstract

We demonstrate a flow cytometer in which structured light illumination is used to attribute fluorescent and scattering signals to their excitation wavelength. A suitable multi-color light source emitting structured illumination patterns at 405, 488, 561 and 640 nm is developed based on a silicon nitride photonic integrated circuit and cytometry experiments are conducted with calibration beads. Performance metrics of the novel cytometer are compared with those of a mature, commercial device. While the experimental device still features a slightly higher sensitivity floor than the commercial one, all but the most weakly stained beads can be categorized. These promising results validate the feasibility of the proposed concept.

Authors 11

1. Alireza Tabatabaei Mashayekh Aachen Institute of Integrated Photonics (IPH)

   RWTH Aachen University

   Affiliation as printed

   Institute of Integrated Photonics, RWTH Aachen University, Campus Blvd. 73, 52074 Aachen, Germany

2. Katharina von Schoeler

   Miltenyi Biotec (Germany)

   Affiliation as printed

   Miltenyi Biotec

3. Manuel Ackermann Aachen Institute of Integrated Photonics (IPH)

   RWTH Aachen University

   Affiliation as printed

   Institute of Integrated Photonics, RWTH Aachen University, Campus Blvd. 73, 52074 Aachen, Germany

4. Thomas Klos

   Toptica Photonics (Germany)

   Affiliation as printed

   TOPTICA Photonics AG

5. Douwe H. Geuzebroek

   LioniX (Netherlands)

   Affiliation as printed

   LioniX International BV

6. Edwin Jan Klein

   LioniX (Netherlands)

   Affiliation as printed

   LioniX International BV

7. T.T. Veenstra

   LioniX (Netherlands)

   Affiliation as printed

   LioniX International BV

8. Ronald Dekker

   LioniX (Netherlands)

   Affiliation as printed

   LioniX International BV

9. Florian Merget Aachen Institute of Integrated Photonics (IPH)

   RWTH Aachen University

   Affiliation as printed

   Institute of Integrated Photonics, RWTH Aachen University, Campus Blvd. 73, 52074 Aachen, Germany

10. P. Leisching

    Toptica Photonics (Germany)

    Affiliation as printed

    TOPTICA Photonics AG

11. Jeremy Witzens Aachen Institute of Integrated Photonics (IPH)

    RWTH Aachen University

    Affiliation as printed

    Institute of Integrated Photonics, RWTH Aachen University, Campus Blvd. 73, 52074 Aachen, Germany