Enhancing Robustness of Sortase A by Loop Engineering and Backbone Cyclization

Chemistry - A European Journal, vol. 26, pp. 13537

Abstract

Abstract Invited for the cover of this issue is Ulrich Schwaneberg and co‐workers at RWTH Aachen University and DWI Leibniz‐Institut für Interaktive Materialien. The image depicts a loop engineered, and backbone cyclized Staphylococcus aureus sortase A which shows enhanced robustness in site‐specific protein and peptide modifications. Read the full text of the article at 10.1002/chem.202002740 .

Authors 5

1. Zhi Zou Aachen Institute of Biotechnology

   DWI – Leibniz Institute for Interactive Materials · RWTH Aachen University

   Affiliation as printed

   DWI–Leibniz-Institute for Interactive Materials Forckenbeckstraβe 50 52074 Aachen Germany

   Institute of Biotechnology RWTH Aachen University Worringerweg 3 52074 Aachen Germany

   Institute of Biotechnology, RWTH Aachen University, Worringerweg 3, 52074 Aachen, Germany

2. Diana M. Maté Aachen

   DWI – Leibniz Institute for Interactive Materials · Centro de Biología Molecular Severo Ochoa · Universidad Autónoma de Madrid

   Affiliation as printed

   Current address: Center of Molecular Biology “Severo Ochoa” Universidad Autónoma de Madrid Nicolás Cabrera 1 28049 Madrid Spain

   DWI–Leibniz-Institute for Interactive Materials Forckenbeckstraβe 50 52074 Aachen Germany

3. Maximilian Nöth Aachen Institute of Biotechnology

   DWI – Leibniz Institute for Interactive Materials · RWTH Aachen University

   Affiliation as printed

   DWI–Leibniz-Institute for Interactive Materials Forckenbeckstraβe 50 52074 Aachen Germany

   Institute of Biotechnology RWTH Aachen University Worringerweg 3 52074 Aachen Germany

   Institute of Biotechnology, RWTH Aachen University, Worringerweg 3, 52074 Aachen, Germany

4. Felix Jakob Aachen Institute of Biotechnology

   DWI – Leibniz Institute for Interactive Materials · RWTH Aachen University

   Affiliation as printed

   DWI–Leibniz-Institute for Interactive Materials Forckenbeckstraβe 50 52074 Aachen Germany

   Institute of Biotechnology RWTH Aachen University Worringerweg 3 52074 Aachen Germany

   Institute of Biotechnology, RWTH Aachen University, Worringerweg 3, 52074 Aachen, Germany

5. Ulrich Schwaneberg corresponding Aachen Institute of Biotechnology

   DWI – Leibniz Institute for Interactive Materials · RWTH Aachen University

   Affiliation as printed

   DWI–Leibniz-Institute for Interactive Materials Forckenbeckstraβe 50 52074 Aachen Germany

   Institute of Biotechnology RWTH Aachen University Worringerweg 3 52074 Aachen Germany

   Institute of Biotechnology, RWTH Aachen University, Worringerweg 3, 52074 Aachen, Germany