Benzoate synthesis from glucose or glycerol using engineered Pseudomonas taiwanensis

Abstract

Benzoic acid is one of the most commonly used food preservatives, but currently exclusively produced in petrochemical processes. In this study, we describe a bio-based production pathway using an engineered strain of Pseudomonas taiwanensis . In a phenylalanine-overproducing strain, we heterologously expressed bacterial, yeast, and plant genes to achieve production of benzoate via a β-oxidation pathway. Strategic disruption of the native Pseudomonas benzoate degradation pathway further allowed the production of catechol and cis , cis -muconate. Taken together, this work demonstrates new routes for the microbial production of these industrially relevant chemicals from renewable resources.

Authors 5

1. Maike Otto

   Forschungszentrum Jülich

   Affiliation as printed

   Forschungszentrum Jülich GmbH

2. Benedikt Wynands

   Forschungszentrum Jülich

   Affiliation as printed

   Forschungszentrum Julich GmbH

3. Jan Marienhagen

   Forschungszentrum Jülich

   Affiliation as printed

   Forschungszentrum Julich GmbH

4. Lars M. Blank Aachen

   RWTH Aachen University

   Affiliation as printed

   Rheinisch Westfalische Technische Hochschule Aachen

5. Nick J. P. Wierckx

   Forschungszentrum Jülich

   Affiliation as printed

   Forschungszentrum Jülich GmbH