Progress and perspectives in single-molecule optical spectroscopy

The Journal of Chemical Physics, vol. 156, pp. 160903

Abstract

We review some of the progress of single-molecule optical experiments in the past 20 years and propose some perspectives for the coming years. We particularly focus on methodological advances in fluorescence, super-resolution, photothermal contrast, and interferometric scattering and briefly discuss a few of the applications. These advances have enabled the exploration of new emitters and quantum optics; the chemistry and biology of complex heterogeneous systems, nanoparticles, and plasmonics; and the detection and study of non-fluorescing and non-absorbing nano-objects. We conclude by proposing some ideas for future experiments. The field will move toward more and better signals of a broader variety of objects and toward a sharper view of the surprising complexity of the nanoscale world of single (bio-)molecules, nanoparticles, and their nano-environments.

Authors 2

1. Subhasis Adhikari Aachen Huygens-Kamerlingh Onnes Laboratory

   Leiden University

   Affiliation as printed

   Huygens-Kamerlingh Onnes Laboratory, Leiden University , P.O. Box 9504, 2333 CA Leiden, The Netherlands

2. Michel Orrit Aachen Huygens-Kamerlingh Onnes Laboratory

   Leiden University

   Affiliation as printed

   Huygens-Kamerlingh Onnes Laboratory, Leiden University , P.O. Box 9504, 2333 CA Leiden, The Netherlands