Postcolumn Infusion as a Quality Control Tool for LC-MS-Based Analysis

Journal of the American Society for Mass Spectrometry, vol. 33, pp. 1077–1080

Abstract

Postcolumn infusion has been widely used to study the matrix effect of analytical methods based on liquid chromatography coupled to mass spectrometry (LC-MS). Nevertheless, this methodology is usually only applied during a method development or validation. With this application note, we aim to demonstrate that the continuous use of postcolumn infusion can be also a very useful tool to monitor the quality of LC-MS analyses and easily detect flaws in the analytical method performance. Here we propose a protocol that can be transferred to other LC-MS platforms, and we show some real situations in bioanalysis in which postcolumn infusion proved to be extremely helpful in, for example, the evaluation of a sample treatment or the detection of unexpected sources of the matrix effect.

Authors 3

1. Oskar González corresponding Aachen Analytical Biosciences and Metabolomics Leiden Academic Centre for Drug Research

   Leiden University

   Affiliation as printed

   Analytical BioSciences and Metabolomics, Leiden Academic Centre for Drug Research, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands

2. Anne-Charlotte Dubbelman Aachen Analytical Biosciences and Metabolomics Leiden Academic Centre for Drug Research

   Leiden University

   Affiliation as printed

   Analytical BioSciences and Metabolomics, Leiden Academic Centre for Drug Research, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands

3. Thomas Hankemeier Aachen Analytical Biosciences and Metabolomics Leiden Academic Centre for Drug Research

   Leiden University

   Affiliation as printed

   Analytical BioSciences and Metabolomics, Leiden Academic Centre for Drug Research, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands