Theory of defect-mediated morphogenesis

Science Advances, vol. 8, pp. eabk2712

Abstract

Growing experimental evidence indicates that topological defects could serve as organizing centers in the morphogenesis of tissues. Here, we provide a quantitative explanation for this phenomenon, rooted in the buckling theory of deformable active polar liquid crystals. Using a combination of linear stability analysis and computational fluid dynamics, we demonstrate that active layers, such as confined cell monolayers, are unstable to the formation of protrusions in the presence of disclinations. The instability originates from an interplay between the focusing of the elastic forces, mediated by defects, and the renormalization of the system's surface tension by the active flow. The posttransitional regime is also characterized by several complex morphodynamical processes, such as oscillatory deformations, droplet nucleation, and active turbulence. Our findings offer an explanation of recent observations on tissue morphogenesis and shed light on the dynamics of active surfaces in general.

Authors 4

1. Ludwig A. Hoffmann Aachen Instituut-Lorentz

   Leiden University

   Affiliation as printed

   Instituut-Lorentz, Universiteit Leiden, P.O. Box 9506, 2300 RA, Leiden, Netherlands

2. Livio Nicola Carenza Aachen Instituut-Lorentz

   Leiden University

   Affiliation as printed

   Instituut-Lorentz, Universiteit Leiden, P.O. Box 9506, 2300 RA, Leiden, Netherlands

3. Julia Eckert Aachen Physics of Life Processes

   Leiden University

   Affiliation as printed

   Physics of Life Processes, Leiden Institute of Physics, Universiteit Leiden, P.O. Box 9506, 2300 RA, Leiden, Netherlands

4. Luca Giomi corresponding Aachen Instituut-Lorentz

   Leiden University

   Affiliation as printed

   Instituut-Lorentz, Universiteit Leiden, P.O. Box 9506, 2300 RA, Leiden, Netherlands