A Novel Bacteriophage with Broad Host Range against Clostridioides difficile Ribotype 078 Supports SlpA as the Likely Phage Receptor
Microbiology Spectrum, vol. 10, pp. e0229521
Abstract
Research into phage therapy has seen a resurgence in recent years owing to growing concerns regarding antimicrobial resistance. Phage research for potential therapy against Clostridioides difficile infection (CDI) is in its infancy, where an optimal "one size fits all" phage cocktail is yet to be derived. The pursuit thus far has aimed to find phages with the broadest possible host range. However, for C. difficile strains belonging to certain PCR ribotypes (RTs), in particular RT 078, phages with broad host range activity are yet to be discovered. In this study, we isolate four novel myoviruses, including ΦCD1801, which exerts the broadest host range activity toward RT 078 reported in the literature. Through the application of ΦCD1801 to phage-binding assays, we provide data to support the prior notion that SlpA represents the likely phage receptor on the bacterial cell surface. Our finding directs research attention toward the isolation of phages with activity toward strains possessing defined S-layer cassette types.
Authors 7
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Engineering and Physical Sciences Research Council · University of Nottingham · Biotechnology and Biological Sciences Research Council
Affiliation as printed
Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Biodiscovery Institute, The University of Nottingham, Nottingham, United Kingdom
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Engineering and Physical Sciences Research Council · University of Nottingham · Biotechnology and Biological Sciences Research Council
Affiliation as printed
Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Biodiscovery Institute, The University of Nottingham, Nottingham, United Kingdom
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Leiden University · Leiden University Medical Center
Affiliation as printed
Faculty of Medicine, Leiden University Medical Centre, the Netherlands
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Medizinische Hochschule Hannover
Affiliation as printed
Hannover Medical School, Hannover, Germany
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Engineering and Physical Sciences Research Council · Nottingham University Hospitals NHS Trust · University of Nottingham · Biotechnology and Biological Sciences Research Council · Nottingham Biomedical Research Centre
Affiliation as printed
Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Biodiscovery Institute, The University of Nottingham, Nottingham, United Kingdom
NIHR Nottingham Biomedical Research Centre, Nottingham University Hospitals NHS Trust and The University of Nottingham, Nottingham, United Kingdom
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Engineering and Physical Sciences Research Council · Nottingham University Hospitals NHS Trust · University of Nottingham · Biotechnology and Biological Sciences Research Council · Nottingham Biomedical Research Centre
Affiliation as printed
Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Biodiscovery Institute, The University of Nottingham, Nottingham, United Kingdom
NIHR Nottingham Biomedical Research Centre, Nottingham University Hospitals NHS Trust and The University of Nottingham, Nottingham, United Kingdom
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Nigel Peter Minton corresponding
Engineering and Physical Sciences Research Council · Nottingham University Hospitals NHS Trust · University of Nottingham · Biotechnology and Biological Sciences Research Council · Nottingham Biomedical Research Centre
Affiliation as printed
Clostridia Research Group, BBSRC/EPSRC Synthetic Biology Research Centre (SBRC), School of Life Sciences, Biodiscovery Institute, The University of Nottingham, Nottingham, United Kingdom
NIHR Nottingham Biomedical Research Centre, Nottingham University Hospitals NHS Trust and The University of Nottingham, Nottingham, United Kingdom
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