SPI2 T3SS effectors facilitate enterocyte apical to basolateral transmigration of Salmonella -containing vacuoles in vivo

Gut Microbes, vol. 13, pp. 1973836

Abstract

Salmonella pathogenicity island (SPI) 2 type three secretion system (T3SS)-mediated effector molecules facilitate bacterial survival in phagocytes but their role in the intestinal epithelium in vivo remains ill-defined. Using our neonatal murine infection model in combination with SPI2 reporter technology and RNA-Seq of sorted primary enterocytes, we demonstrate expression of SPI2 effector molecules by intraepithelial Salmonella Typhimurium (S. Typhimurium). Contrary to expectation, immunostaining revealed that infection with SPI2 T3SS-mutants resulted in significantly enlarged intraepithelial Salmonella-containing vacuoles (SCV) with altered cellular positioning, suggesting impaired apical to basolateral transmigration. Also, infection with isogenic tagged S. Typhimurium strains revealed a reduced spread of intraepithelial SPI2 T3SS mutant S. Typhimurium to systemic body sites. These results suggest that SPI2 T3SS effector molecules contribute to enterocyte apical to basolateral transmigration of the SCV during the early stage of the infection.

Authors 18

1. Marcus Fulde corresponding
   Affiliation as printed

   Institute of Microbiology and Epizootics

2. Kira van Vorst
   Affiliation as printed

   Institute of Microbiology and Epizootics

3. Kaiyi Zhang Aachen Institute of Medical Microbiology

   Universitätsklinikum Aachen · RWTH Aachen University

   Affiliation as printed

   Institute of Medical Microbiology, Rwth University Hospital Aachen

   Institute of Medical Microbiology, Rwth University Hospital Aachen, Aachen, Germany

4. Alexander J. Westermann

   University of Würzburg · Helmholtz Centre for Infection Research · Helmholtz Institute for RNA-based Infection Research

   Affiliation as printed

   Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI)

   Institute of Molecular Infection Biology (IMIB), University of Würzburg

5. Tobias Busche

   Bielefeld University

   Affiliation as printed

   Center for Biotechnology (Cebitec), Bielefeld University

   Technology Platform Genomics, Center for Biotechnology (Cebitec), Bielefeld University, Bielefeld, Germany

6. Yong Chiun Huei Aachen Institute of Medical Microbiology

   Universitätsklinikum Aachen · RWTH Aachen University

   Affiliation as printed

   Institute of Medical Microbiology, Rwth University Hospital Aachen

   Institute of Medical Microbiology, Rwth University Hospital Aachen, Aachen, Germany

7. Katharina Welitschanski

   Medizinische Hochschule Hannover

   Affiliation as printed

   Institute for Medical Microbiology and Hospital Epidemiology

   Hannover Medical School, Institute for Medical Microbiology and Hospital Epidemiology, Hannover, Germany

8. Isabell Froh

   Medizinische Hochschule Hannover

   Affiliation as printed

   Institute for Medical Microbiology and Hospital Epidemiology

   Hannover Medical School, Institute for Medical Microbiology and Hospital Epidemiology, Hannover, Germany

9. Dennis Pägelow
   Affiliation as printed

   Institute of Microbiology and Epizootics

10. Johanna Plendl
    Affiliation as printed

    Institute of Veterinary Anatomy

11. Christiane D. Pfarrer

    University of Veterinary Medicine Hannover, Foundation

    Affiliation as printed

    University of Veterinary Medicine Hannover, Foundation

    Institute for Anatomy, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany

12. Jörn Kalinowski

    Bielefeld University

    Affiliation as printed

    Center for Biotechnology (Cebitec), Bielefeld University

    Technology Platform Genomics, Center for Biotechnology (Cebitec), Bielefeld University, Bielefeld, Germany

13. Jörg Vogel

    University of Würzburg · Helmholtz Centre for Infection Research · Helmholtz Institute for RNA-based Infection Research

    Affiliation as printed

    Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI)

    Institute of Molecular Infection Biology (IMIB), University of Würzburg

14. Peter Valentin‐Weigand

    University of Veterinary Medicine Hannover, Foundation

    Affiliation as printed

    Institute of Microbiology, University of Veterinary Medicine Hannover, Foundation

    Institute of Microbiology, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany

15. Michael Hensel

    Osnabrück University

    Affiliation as printed

    University of Osnabrück

16. Karsten Tedin
    Affiliation as printed

    Institute of Microbiology and Epizootics

17. Urška Repnik

    University of Oslo

    Affiliation as printed

    University of Oslo

    Department of Biosciences, University of Oslo, Oslo, Norway

18. Mathias Walter Hornef Aachen Institute of Medical Microbiology

    Universitätsklinikum Aachen · RWTH Aachen University

    Affiliation as printed

    Institute of Medical Microbiology, Rwth University Hospital Aachen

    Institute of Medical Microbiology, Rwth University Hospital Aachen, Aachen, Germany