EEG-based visual deviance detection in freely behaving mice

bioRxiv (Cold Spring Harbor Laboratory)

Abstract

Abstract The mouse is widely used as an experimental model to study visual processing. To probe how the visual system detects changes in the environment, functional paradigms in freely behaving mice are strongly needed. We developed and validated the first EEG-based method to investigate visual deviance detection in freely behaving mice. Mice with EEG implants were exposed to a visual deviant detection paradigm that involved changes in light intensity as standard and deviant stimuli. By subtracting the standard from the deviant evoked waveform, deviant detection was evident as bi-phasic negativity (starting around 70 ms) in the difference waveform. Additionally, deviance-associated evoked (beta/gamma) and induced (gamma) oscillatory responses were found. We showed that the results were stimulus-independent by applying a “flip-flop” design and the results showed good repeatability in an independent measurement. Together, we put forward a validated, easy-to-use paradigm to measure visual deviance processing in freely behaving mice.

Authors 8

1. Renate Kat corresponding

   University of Groningen

   Affiliation as printed

   Groningen Institute for Evolutionary Life Sciences (GELIFES), University of Groningen, Nijenborgh 7, 9747 AG, Groningen, the Netherlands, ,

   Groningen Institute for Evolutionry Life Sciences (GELIFES), University of Groningen, Nijenborgh 7, 9747 AG, Groningen, the Netherlnds, ,

2. Berry van den Berg corresponding

   University of Groningen

   Affiliation as printed

   Faculty of Behavioural and Social Sciences, Cognitive Neuroscience, Department of Experimental Psychology, University of Groningen, Grote Kruisstraat 2/1, 9712 TS, Groningen, the Netherlands,

3. Matthijs Perenboom corresponding Aachen Department of Neurology, Leiden University Medical Center (LUMC) Department of Neurology, Leiden University Medial Center

   Leiden University · Leiden University Medical Center

   Affiliation as printed

   Department of Neurology, Leiden University Medical Center, Albinusdreef 2, 2300 RC, Leiden, the Netherlands, , ,

   Department of Neurology, Leiden University Medial Center, Albinusdreef 2, 2300 RC, Leiden, the Netherlands, , ,

4. Maarten Schenke Aachen Department of Human Genetics

   Leiden University Medical Center

   Affiliation as printed

   Department of Human Genetics, Leiden University Medical Center, Einthovenweg 20, 2300 RC, Leiden, the Netherlands

   Department of Human Genetics, Leien University Meical Center, Einthovenweg 20, 2300 RC, Leien, the Netherlans

5. Arn M. J. M. van den Maagdenberg corresponding Aachen Department of Human Genetics Department of Neurology, Leiden University Medical Center (LUMC) Department of Neurology, Leiden University Medial Center

   Leiden University · Leiden University Medical Center

   Affiliation as printed

   Department of Human Genetics, Leiden University Medical Center, Einthovenweg 20, 2300 RC, Leiden, the Netherlands

   Department of Neurology, Leiden University Medical Center, Albinusdreef 2, 2300 RC, Leiden, the Netherlands, , ,

   Department of Human Genetics, Leien University Meical Center, Einthovenweg 20, 2300 RC, Leien, the Netherlans

   Department of Neurology, Leiden University Medial Center, Albinusdreef 2, 2300 RC, Leiden, the Netherlands, , ,

6. Hilgo Bruining corresponding

   Amsterdam University Medical Centers · University of Amsterdam

   Affiliation as printed

   Department of Child and Adolescent Psychiatry, Amsterdam University Medical Center, University of Amsterdam, Postbus 7057, 1007 MB, Amsterdam, the Netherlands,

   Dpartmnt of Child and Adolscnt Psychiatry, Amstrdam Univrsity Mdical Cntr, Univrsity of Amstrdam, Postbus 7057, 1007 MB, Amstrdam, th Nthrlands,

7. Else A. Tolner corresponding Aachen Department of Human Genetics Department of Neurology, Leiden University Medical Center (LUMC) Department of Neurology, Leiden University Medial Center

   Leiden University · Leiden University Medical Center

   Affiliation as printed

   Department of Human Genetics, Leiden University Medical Center, Einthovenweg 20, 2300 RC, Leiden, the Netherlands

   Department of Neurology, Leiden University Medical Center, Albinusdreef 2, 2300 RC, Leiden, the Netherlands, , ,

   Department of Human Genetics, Leien University Meical Center, Einthovenweg 20, 2300 RC, Leien, the Netherlans

   Department of Neurology, Leiden University Medial Center, Albinusdreef 2, 2300 RC, Leiden, the Netherlands, , ,

8. Martien J. Kas corresponding

   University of Groningen

   Affiliation as printed

   Groningen Institute for Evolutionary Life Sciences (GELIFES), University of Groningen, Nijenborgh 7, 9747 AG, Groningen, the Netherlands, ,

   Groningen Institute for Evolutionry Life Sciences (GELIFES), University of Groningen, Nijenborgh 7, 9747 AG, Groningen, the Netherlnds, ,