Adaptive Laboratory Evolution Restores Solvent Tolerance in Plasmid-Cured Pseudomonas putida S12: a Molecular Analysis

Applied and Environmental Microbiology, vol. 87

Abstract

Sustainable production of high-value chemicals can be achieved by bacterial biocatalysis. However, bioproduction of biopolymers and aromatic compounds may exert stress on the microbial production host and limit the resulting yield.

Authors 7

1. Hadiastri Kusumawardhani Aachen Institute of Biology Leiden

   Leiden University

   Affiliation as printed

   Institute of Biology Leiden, Leiden University, Leiden, The Netherlands

2. Benjamin Furtwängler Aachen Institute of Biology Leiden

   Leiden University

   Affiliation as printed

   Institute of Biology Leiden, Leiden University, Leiden, The Netherlands

3. Matthijs Blommestijn Aachen Institute of Biology Leiden

   Leiden University

   Affiliation as printed

   Institute of Biology Leiden, Leiden University, Leiden, The Netherlands

4. Adelė Kaltenytė Aachen Institute of Biology Leiden

   Leiden University

   Affiliation as printed

   Institute of Biology Leiden, Leiden University, Leiden, The Netherlands

5. Jaap van der Poel Aachen Institute of Biology Leiden

   Leiden University

   Affiliation as printed

   Institute of Biology Leiden, Leiden University, Leiden, The Netherlands

6. Rohola Hosseini Aachen Institute of Biology Leiden

   Leiden University

   Affiliation as printed

   Institute of Biology Leiden, Leiden University, Leiden, The Netherlands

7. Johannes H. DE WINDE corresponding Aachen Institute of Biology Leiden

   Leiden University

   Affiliation as printed

   Institute of Biology Leiden, Leiden University, Leiden, The Netherlands