Front Cover: Enhancing Robustness of Sortase A by Loop Engineering and Backbone Cyclization (Chem. Eur. J. 60/2020)

Chemistry - A European Journal, vol. 26, pp. 13533

Abstract

Sortase-mediated ligation (sortagging) has emerged as one of the most popular methodologies for site-specific modification of proteins. The cover image shows a loop engineering and subsequent head-to-tail backbone cyclization of Staphylococcus aureus sortase A (SaSrtA, the engineered loop and fused termini are highlighted in cyan). The reengineered SaSrtA has an enhanced robustness towards thermal and chemical denaturation. More information can be found in the Communication by U. Schwaneberg et al. on page 13568.

Authors 5

1. Zhi Zou Aachen Institute of Biotechnology

   DWI – Leibniz Institute for Interactive Materials · RWTH Aachen University

   Affiliation as printed

   DWI–Leibniz-Institute for Interactive Materials Forckenbeckstraβe 50 52074 Aachen Germany

   Institute of Biotechnology RWTH Aachen University Worringerweg 3 52074 Aachen Germany

   Institute of Biotechnology, RWTH Aachen University, Worringerweg 3, 52074 Aachen, Germany

2. Diana M. Maté Aachen

   DWI – Leibniz Institute for Interactive Materials · Centro de Biología Molecular Severo Ochoa · Universidad Autónoma de Madrid

   Affiliation as printed

   Current address: Center of Molecular Biology “Severo Ochoa” Universidad Autónoma de Madrid Nicolás Cabrera 1 28049 Madrid Spain

   DWI–Leibniz-Institute for Interactive Materials Forckenbeckstraβe 50 52074 Aachen Germany

3. Maximilian Nöth Aachen Institute of Biotechnology

   DWI – Leibniz Institute for Interactive Materials · RWTH Aachen University

   Affiliation as printed

   DWI–Leibniz-Institute for Interactive Materials Forckenbeckstraβe 50 52074 Aachen Germany

   Institute of Biotechnology RWTH Aachen University Worringerweg 3 52074 Aachen Germany

   Institute of Biotechnology, RWTH Aachen University, Worringerweg 3, 52074 Aachen, Germany

4. Felix Jakob Aachen Institute of Biotechnology

   DWI – Leibniz Institute for Interactive Materials · RWTH Aachen University

   Affiliation as printed

   DWI–Leibniz-Institute for Interactive Materials Forckenbeckstraβe 50 52074 Aachen Germany

   Institute of Biotechnology RWTH Aachen University Worringerweg 3 52074 Aachen Germany

   Institute of Biotechnology, RWTH Aachen University, Worringerweg 3, 52074 Aachen, Germany

5. Ulrich Schwaneberg corresponding Aachen Institute of Biotechnology

   DWI – Leibniz Institute for Interactive Materials · RWTH Aachen University

   Affiliation as printed

   DWI–Leibniz-Institute for Interactive Materials Forckenbeckstraβe 50 52074 Aachen Germany

   Institute of Biotechnology RWTH Aachen University Worringerweg 3 52074 Aachen Germany

   Institute of Biotechnology, RWTH Aachen University, Worringerweg 3, 52074 Aachen, Germany