Bioactive Three-Dimensional Graphene Oxide Foam/Polydimethylsiloxane/Zinc Silicate Scaffolds with Enhanced Osteoinductivity for Bone Regeneration
ACS Biomaterials Science & Engineering, vol. 6, pp. 3015–3025
Abstract
Nanocomposite scaffold materials have shown great prospect in promoting bone integration and bone regeneration. A three-dimensional graphene oxide foam/polydimethylsiloxane/zinc silicate (GF/PDMS/ZS) scaffold for bone tissue engineering was synthesized via dip coating and hydrothermal synthesis processes, resulting in the interconnected macroporous structure. The scaffold was characterized with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and thermogravimetric (TG) analysis. The result showed that scaffolds exhibiting a porous characteristic had organic–inorganic components similar to natural bone tissue. Moreover, the scaffolds possessed suitable pore size, high porosity, and good mechanical properties. In vitro experiments with mouse bone marrow mesenchymal stem cells (mBMSCs) revealed that the composite scaffold not only has great biocompatibility but also has the ability to induce mBMSC proliferation and preferential osteogentic differentiation. Thereafter, the expression of critical genes, ALP, RUNX2, VEGFA, and OPN, was activated. In vivo analysis of critical bone defect in rabbits demonstrated superior bone formation in defect sites in the GF/PDMS/ZS scaffold group at 12 weeks of post implantation without no significant inflammatory response. All the results validated that the GF/PDMS/ZS scaffold is a promising alternative for applications in bone regeneration.
Authors 11
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Xuzhou Medical College · Affiliated Hospital of Xuzhou Medical College
Affiliation as printed
Department of Orthopedics, Affiliated Hospital of Xuzhou Medical University, 221002, Xuzhou, China
School of Pharmacy, Xuzhou Medical University, 221004, Xuzhou, China
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Universitätsklinikum Aachen · RWTH Aachen University
Affiliation as printed
Department of Trauma and Reconstructive Surgery, RWTH Aachen University Hospital, Aachen 52074, Germany
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Xuzhou Medical College · Affiliated Hospital of Xuzhou Medical College
Affiliation as printed
Department of Orthopedics, Affiliated Hospital of Xuzhou Medical University, 221002, Xuzhou, China
School of Pharmacy, Xuzhou Medical University, 221004, Xuzhou, China
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Affiliation as printed
School of Pharmacy, Xuzhou Medical University, 221004, Xuzhou, China
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Affiliation as printed
School of Pharmacy, Xuzhou Medical University, 221004, Xuzhou, China
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Xuzhou Medical College · Affiliated Hospital of Xuzhou Medical College
Affiliation as printed
Department of Orthopedics, Affiliated Hospital of Xuzhou Medical University, 221002, Xuzhou, China
School of Pharmacy, Xuzhou Medical University, 221004, Xuzhou, China
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Xuzhou Medical College · Affiliated Hospital of Xuzhou Medical College
Affiliation as printed
Department of Orthopedics, Affiliated Hospital of Xuzhou Medical University, 221002, Xuzhou, China
School of Pharmacy, Xuzhou Medical University, 221004, Xuzhou, China
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Xuzhou Medical College · Affiliated Hospital of Xuzhou Medical College
Affiliation as printed
Department of Orthopedics, Affiliated Hospital of Xuzhou Medical University, 221002, Xuzhou, China
School of Pharmacy, Xuzhou Medical University, 221004, Xuzhou, China
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Affiliation as printed
School of Pharmacy, Xuzhou Medical University, 221004, Xuzhou, China
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Kaijin Guo corresponding
Xuzhou Medical College · Affiliated Hospital of Xuzhou Medical College
Affiliation as printed
Department of Orthopedics, Affiliated Hospital of Xuzhou Medical University, 221002, Xuzhou, China
School of Pharmacy, Xuzhou Medical University, 221004, Xuzhou, China
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Fenglei Gao corresponding
Affiliation as printed
School of Pharmacy, Xuzhou Medical University, 221004, Xuzhou, China
Cited by 39 stored of 39
Cited by patents worldwide 1 (Lens.org)
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