Engineered Near‐Infrared Fluorescent Protein Assemblies for Robust Bioimaging and Therapeutic Applications
Advanced Materials, vol. 32, pp. e2000964
Abstract
Fluorescent proteins are investigated extensively as markers for the imaging of cells and tissues that are treated by gene transfection. However, limited transfection efficiency and lack of targeting restrict the clinical application of this method rooted in the challenging development of robust fluorescent proteins for in vivo bioimaging. To address this, a new type of near-infrared (NIR) fluorescent protein assemblies manufactured by genetic engineering is presented. Due to the formation of well-defined nanoparticles and spectral operation within the phototherapeutic window, the NIR protein aggregates allow stable and specific tumor imaging via simple exogenous injection. Importantly, in vivo tumor metastases are tracked and this overcomes the limitations of in vivo imaging that can only be implemented relying on the gene transfection of fluorescent proteins. Concomitantly, the efficient loading of hydrophobic drugs into the protein nanoparticles is demonstrated facilitating the therapy of tumors in a mouse model. It is believed that these theranostic NIR fluorescent protein assemblies, hence, show great potential for the in vivo detection and therapy of cancer.
Authors 10
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Chinese Academy of Sciences · Changchun Institute of Applied Chemistry · State Key Laboratory of Rare Earth Resources Utilization
Affiliation as printed
State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China
State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 China
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Chinese Academy of Sciences · Changchun Institute of Applied Chemistry · State Key Laboratory of Rare Earth Resources Utilization
Affiliation as printed
State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China
State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 China
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University of Groningen · Chinese Academy of Sciences · Changchun Institute of Applied Chemistry · State Key Laboratory of Rare Earth Resources Utilization · Zernike Institute for Advanced Materials
Affiliation as printed
State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China
Zernike Institute for Advanced Materials University of Groningen Nijenborgh 4 Groningen 9747 AG The Netherlands
State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 China
Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, Groningen, 9747 AG The Netherlands
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University of Groningen · Zernike Institute for Advanced Materials
Affiliation as printed
Zernike Institute for Advanced Materials University of Groningen Nijenborgh 4 Groningen 9747 AG The Netherlands
Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, Groningen, 9747 AG The Netherlands
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Chinese Academy of Sciences · Changchun Institute of Applied Chemistry · State Key Laboratory of Rare Earth Resources Utilization
Affiliation as printed
State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China
State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 China
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Chinese Academy of Sciences · Changchun Institute of Applied Chemistry · State Key Laboratory of Rare Earth Resources Utilization
Affiliation as printed
State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China
State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 China
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Robert Göstl Aachen
DWI – Leibniz Institute for Interactive Materials
Affiliation as printed
DWI–Leibniz Institute for Interactive Materials Forckenbeckstr. 50 Aachen 52056 Germany
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University of Groningen · DWI – Leibniz Institute for Interactive Materials · Zernike Institute for Advanced Materials · RWTH Aachen University
Affiliation as printed
DWI–Leibniz Institute for Interactive Materials Forckenbeckstr. 50 Aachen 52056 Germany
Institute of Technical and Macromolecular Chemistry RWTH Aachen University Worringerweg 1 Aachen 52074 Germany
Zernike Institute for Advanced Materials University of Groningen Nijenborgh 4 Groningen 9747 AG The Netherlands
Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Worringerweg 1, Aachen, 52074 Germany
Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, Groningen, 9747 AG The Netherlands
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Kai Liu corresponding
Chinese Academy of Sciences · Changchun Institute of Applied Chemistry · State Key Laboratory of Rare Earth Resources Utilization · Tsinghua University
Affiliation as printed
Department of Chemistry Tsinghua University Beijing 100084 China
State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China
Department of Chemistry, Tsinghua University, Beijing, 100084 China
State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 China
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Chinese Academy of Sciences · Changchun Institute of Applied Chemistry · State Key Laboratory of Rare Earth Resources Utilization · Tsinghua University
Affiliation as printed
Department of Chemistry Tsinghua University Beijing 100084 China
State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China
Department of Chemistry, Tsinghua University, Beijing, 100084 China
State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 China
Cited by 75 stored of 75
Cited by patents worldwide 2 (Lens.org)
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