Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes

• Capturing intrinsic protein dynamics for explaining beneficial substitutions from protein engineering campaigns

  2025 Biotechnology Advances article Biochemistry, Genetics and Molecular Biology Protein Structure and Dynamics

  5

  citations
• KnowVolution of an Efficient Polyamidase through Molecular Dynamics Simulations of Incrementally Docked Oligomeric Substrates

  2025 ChemSusChem article Biochemistry, Genetics and Molecular Biology Enzyme Catalysis and Immobilization Open access

  8

  citations
• KnowVolution of an efficient polyamidase through molecular dynamics simulations of incrementally docked oligomeric substrates

  2024 bioRxiv (Cold Spring Harbor Laboratory) preprint Biochemistry, Genetics and Molecular Biology Enzyme Catalysis and Immobilization Open access

  1

  citations
• Corner Engineering: Tailoring Enzymes for Enhanced Resistance and Thermostability in Deep Eutectic Solvents

  2023 Angewandte Chemie International Edition article Biochemistry, Genetics and Molecular Biology Enzyme Catalysis and Immobilization

  31

  citations
• Corner Engineering: Tailoring Enzymes for Enhanced Resistance and Thermostability in Deep Eutectic Solvents

  2023 Angewandte Chemie article Biochemistry, Genetics and Molecular Biology Enzyme Catalysis and Immobilization

  8

  citations
• Engineering a new-to-nature cascade for phosphate-dependent formate to formaldehyde conversion in vitro and in vivo

  2023 Nature Communications article Biochemistry, Genetics and Molecular Biology Microbial Metabolic Engineering and Bioproduction Open access cited by 2 patents

  50

  citations
• Combinatorial InVitroFlow‐assisted mutagenesis (CombIMut) yields a 41‐fold improved CelA2 cellulase

  2022 Biotechnology and Bioengineering article Biochemistry, Genetics and Molecular Biology Enzyme Catalysis and Immobilization Open access cited by 1 patent

  9

  citations
• Protein Engineering by Efficient Sequence Space Exploration Through Combination of Directed Evolution and Computational Design Methodologies

  2021 Protein Engineering other Biochemistry, Genetics and Molecular Biology Enzyme Catalysis and Immobilization

  13

  citations
• An engineered cellobiohydrolase I for sustainable degradation of lignocellulosic biomass

  2021 Biotechnology and Bioengineering article Engineering Biofuel production and bioconversion Open access

  21

  citations
• Can constraint network analysis guide the identification phase of KnowVolution? A case study on improved thermostability of an endo-β-glucanase

  2020 Computational and Structural Biotechnology Journal article Biochemistry, Genetics and Molecular Biology Enzyme Catalysis and Immobilization Open access

  12

  citations
• KnowVolution of a GH5 Cellulase from Penicillium verruculosum to Improve Thermal Stability for Biomass Degradation

  2020 ACS Sustainable Chemistry & Engineering article Engineering Biofuel production and bioconversion

  57

  citations
• How to Engineer Organic Solvent Resistant Enzymes: Insights from Combined Molecular Dynamics and Directed Evolution Study

  2020 ChemCatChem article Biochemistry, Genetics and Molecular Biology Enzyme Catalysis and Immobilization Open access

  88

  citations

12 results