IMPROVED METHOD FOR GENERATING INNER EAR ORGANOIDS
Abstract
The invention relates to a method of generating endolymph-producing epithelial cells and in vitro use of a hedgehog signalling inhibitor for differentiation of inner ear progenitor cells into endolymph-producing epithelial cells; in vitro endolymph-producing epithelial cells and an inner ear organoid comprising endolymph-producing epithelial cells. Also provided is in vitro use of an inner ear organoid in drug discovery; the endolymph-roducing epithelial cells for use in a method of treating diseases of the inner ear; and a method of testing one of more therapeutic agents using these cells or organoids. Lastly, also provided is a kit and an organoid maturation medium.
Inventors 3
- Wouter H. van der Valk Aachen author as filed: van der Valk, Wouter H.
- Heiko Locher Aachen author as filed: Locher, Heiko
- Peter Paul G. van Benthem Aachen author as filed: van Benthem, Peter Paul G.
Inventors are linked to an Aachen author when surname and first name match exactly one author.
Applicants
- ACADEMISCH ZIEKENHUIS LEIDEN (H.O.D.N. LUMC) GBS Leiden
Cited papers 2 in the database
Non-patent literature as cited (2)
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- - VAN DER VALK WOUTER H ET AL: "Building inner ears: recent advances and future challenges for in vitro organoid systems", CELL DEATH & DIFFERENTIATION, vol. 28, no. 1, 14 December 2020 (2020-12-14), pages 24 - 34, XP037332512, ISSN: 1350-9047, DOI: 10.1038/S41418-020-00678-8