MACROPHAGE POLARIZATION ASSAYS
Espacenet
Family 92295778
Abstract
Computer-implemented methods for identifying the macrophage polarization state of one or more macrophage cells in live cell culture, and drug screening methods making use of such methods are provided. The methods include obtaining single cell label-free images obtained using an optical label-free imaging technology, quantifying a plurality of single cell image-derived features, wherein the plurality of single cell image-derived features comprise: one or more intensity features, one or more shape features and one or more texture features; and identifying a macrophage polarization state associated with the single cell image using a trained machine learning classification model, trained to classify cells between a plurality of classes each associated with a different macrophage polarization state selected from: M0, M1, M2, and a plurality of M2 subtypes.
Inventors 8
- Chatrian, Andrea
- Sörmann Paulsson, Elsa
- Keselman, Paul
- Austerjost, Jonas
- Barnes, Kalpana
- Linda van den Berk Aachen author as filed: van der Berk, Linda
- Richard P. Davis Aachen author as filed: Davis, Richard
- Sörmann, Paulsson Elsa
Inventors are linked to an Aachen author when surname and first name match exactly one author.
Applicants
- Sartorius Stedim Data Analytics AB
- Academisch Ziekenhuis Leiden GBS Leiden
Cited papers 6 in the database
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