DEVICE AND METHOD FOR CRYOGENIC ELECTRON MICROSCOPY SAMPLE PREPARATION
Espacenet
Family 84463193
Abstract
Provided herein is a cryogenic electron microscopy sample preparation device that includes a sample support configured to receive a sample grid, a first applicator configured to comprise a first sample material, a second applicator configured to comprise a second sample material, a first gas outlet, a second gas outlet, at least one guard configured to move between a closed configuration and an open configuration, a cooling bath, a control unit, and an image capturing apparatus; and a method of preparing a sample for cryogenic electron microscopy employing the same.
Inventors 3
- Meindert Hugo Lamers Aachen author as filed: Lamers, Meindert
- Fernandez Leiro, Rafael
- Fernandez, Leiro Rafael
Inventors are linked to an Aachen author when surname and first name match exactly one author.
Applicants
- ACADEMISCH ZIEKENHUIS LEIDEN (H.O.D.N. LUMC) GBS Leiden
- FUNDACIÓN DEL SECTOR PÚBLICO ESTATAL CENTRO NACIONAL DE INVESTIGACIONES ONCOLÓGICAS CARLOS III (F.S.P. CNIO)
- Fundacion Del Sector Publico Estatal Centro Nac de Investigaciones Oncologicas Carlos Iii F S P Cnio
Cited papers 2 in the database
Non-patent literature as cited (4)
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- - KONING ROMAN I. ET AL: "Automated vitrification of cryo-EM samples with controllable sample thickness using suction and real-time optical inspection", vol. 13, no. 1, 27 May 2022 (2022-05-27), pages 1 - 10, XP093048760, Retrieved from the Internet <URL:https://www.nature.com/articles/s41467-022-30562-7> DOI: 10.1038/s41467-022-30562-7
- - KLEBL DAVID P. ET AL: "Need for Speed: Examining Protein Behavior during CryoEM Grid Preparation at Different Timescales", STRUCTURE, vol. 28, no. 11, 1 November 2020 (2020-11-01), AMSTERDAM, NL, pages 1238 - 1248.e4, XP093048779, ISSN: 0969-2126, DOI: 10.1016/j.str.2020.07.018
- - KLEBL DAVID P. ET AL: "On-grid and in-flow mixing for time-resolved cryo-EM", ACTA CRYSTALLOGRAPHICA / D. SECTION D, BIOLOGICAL CRYSTALLOGRAPHY, vol. 77, no. 10, 23 August 2021 (2021-08-23), Oxford, pages 1233 - 1240, XP093048273, ISSN: 2059-7983, DOI: 10.1107/S2059798321008810