MULTIPLEXED IMPEDANCE-BASED DETECTION METHODS AND SYSTEMS USING IMPEDANCE-ENCODED PARTICLES
Abstract
Described herein are multiplexed impedance-based detection methods for identifying each type of impedance-encoded particles and systems for performing these methods. Impedance-encoded particles of each type comprise cores having the same structure and producing the same complex electrical impedance signature when an AC signal is applied to these particles. At the same time, different types of particles have different structures and produce different complex electrical impedance signatures, which allow differentiation of the different types of particles. In some examples, different types of particles have different functionalization, resulting in different analytes binding to or otherwise reacting with these particles. As such, these particles may be arranged into a test media to detect different analytes based on identifying each type of impedance-encoded particles using complex electrical impedance signatures. Furthermore, a multiplexed impedance-based detection system may include an optical detector to determine the concentration of these analytes or a particle sorter.
Inventors 2
- Harink, Matthijs Bjorn Marijn
- Bramall, Nathan Earl
Inventors are linked to an Aachen author when surname and first name match exactly one author.
Applicants
- LEIDEN MEASUREMENT TECHNOLOGY LLC GBS Leiden
Cited papers 0 in the database
Non-patent literature as cited (11)
- - Caselli, Federica et al., A novel wiring scheme for standard chips enabling high-accuracy impedance cytometry; Sensors and Actuators B 256 (2018), pp. 580-589.
- - Feng, Yinnian et al., MRBLES 2.0: High-throughput generation of chemically functionalized spectrally and magnetically-encoded hydrogel beads using a simple single-layer microfluidic device; Retrieved from the Internet: https://www.biorxiv.org/content/10.1101/2020.06.22.166074v1, 19 pgs.
- - Gerver, R.E. et al., Programmable microfluidic synthesis of spectrally encoded microspheres, Lab Chip, 2012, 12, pp. 4716-4723.
- - Guo, Jia et al., Poly(N-isopropylacrylamide)-Coated Luminescent/Magnetic Silica Microspheres: Preparation, Characterization, and Biomedical Applications; Chem. Mater. 2006, 18, pp. 5554-5562.
- - Nair, Devatha P. et al., The Thiol-Michael Addition Click Reaction: A Powerful and Widely Used Tool in Materials Chemistry; American Chemical Society 2013, pp. 724-744.
- - Nguyen, Huy Q. et al., Peptide library synthesis on spectrally encoded beads for multiplexed protein/peptide bioassays; Proc. SPIE 10061, Microfluidics, BioMEMS, and Medical Microsystems XV, 100610Z (Feb. 28, 2017), 12 pgs.
- - Nguyen, Huy Quoc et al., Quantitative mapping of protein-peptide affinity landscapes using spectrally encoded beads; eLife 2019;8:e40499, Retrieved from the Internet: DOI: https://doi.org/10.7554/eLife.40499, 28 pgs.
- - Wilson, Robert et al., Magnetic microspheres encoded with photoluminescent quantum dots for multiplexed detection; Journal of Materials Chemistry, 2007, 17, pp. 4400-4406.
- - Xie et al., Top-down fabrication meets bottom-up synthesis for nanoelectronic barcoding of microparticles, Lab Chip, vol. 17 No. 11, pp. 1847-2024.
- - Feng et al., “A Microfluidic Device Integrating Impedance Flow Cytometry and Electric Impedance Spectroscopy for High-Efficiency Single-Cell Electrical Property Measurement”, Anal. Chem., 2019, vol. 91, 15204-15212, published Nov. 8, 2019 (Year: 2019).
- - Prakash et al.,“Design of a Multiplexed Analyte Biosensor using Digital Barcoded Particles and Impedance Spectroscopy”, Scientific Reports, 2020, 10-6109, published 1-10, published Apr. 9, 2020 (Year: 2020).