MEANS AND METHODS FOR DETERMINING MESENCHYMAL STROMAL CELL PROPERTIES.
Abstract
The invention relates to means and method for testing mesenchymal stromal cells (MSC) to determine the quality, potency and/or capacity to inhibit an immune response, such as inhibit proliferation of target cells such as T-cells, B-cells, NK cells and other immune cells and/or monocyte/macrophage polarization resulting in direct immune suppression through the production of soluble mediators and induction of T Regs. Such methods may comprise providing MSC; culturing said MSC in the presence and in the absence of one or more cytokines selected from: interleukin (IL)17A, lL-1β, oncostatin M (OSM), interferon γ (IFN-γ), tumor necrosis factor α (TNF-α), IL-13 and IL-4; contacting said MSC with binding molecules that hind to an MSC -specific marker and one or more selected from: the extracellular markers CD54, CD274, human leukocyte antigen (HLA)-DR, the intracellular markers cyclo oxygenase-2 (COX-2) and indoleamine-pyrrole 2,3-dioxygenase (IDO). Such method may further comprise determining said quality, potency and/or capacity to inhibit on the basis of the expression of said MSC- specific marker and the fold increase of the expression of said one or more of CD54, CD274, HLA-DR, COX-2 and IDO markers in response to said one or more cytokínes. The invention further provides cell banks, kits useful in and for methods of treatment using the tested MSC.
Inventors 3
- Willem E. Fibbe Aachen author as filed: Fibbe, Willem E.
- Melissa van Pel Aachen author as filed: van Pel, Melissa
- Koen Schepers Aachen author as filed: Schepers, Koen
Inventors are linked to an Aachen author when surname and first name match exactly one author.
Applicants
- ACADEMISCH ZIEKENHUIS LEIDEN H.O.D.N. LUMC GBS Leiden
Cited papers 2 in the database
Non-patent literature as cited (5)
- - NÁDIA DE CÁSSIA NORONHA ET AL: "Priming approaches to improve the efficacy of mesenchymal stromal cell-based therapies", STEM CELL RESEARCH & THERAPY, vol. 10, no. 1, 2 May 2019 (2019-05-02), XP055763658, DOI: 10.1186/s13287-019-1224-y
- - GHANNAM S ET AL: "Mesenchymal Stem Cells Inhibit Human Th17 Cell Differentiation and Function and Induce a T Regulatory Cell Phenotype", THE JOURNAL OF IMMUNOLOGY, AMERICAN ASSOCIATION OF IMMUNOLOGISTS, US, vol. 185, no. 1, 1 July 2010 (2010-07-01), pages 302 - 312, XP002612951, ISSN: 0022-1767, [retrieved on 20100528], DOI: 10.4049/JIMMUNOL.0902007
- - NORA G. SINGER ET AL: "Mesenchymal Stem Cells: Mechanisms of Inflammation", ANNUAL REVIEW OF PATHOLOGY: MECHANISMS OF DISEASE, vol. 6, no. 1, 28 February 2011 (2011-02-28), US, pages 457 - 478, XP055763655, ISSN: 1553-4006, DOI: 10.1146/annurev-pathol-011110-130230
- - MARIA ESTER BERNARDO ET AL: "Mesenchymal Stromal Cells: Sensors and Switchers of Inflammation", CELL STEM CELL, vol. 13, no. 4, 1 October 2013 (2013-10-01), AMSTERDAM, NL, pages 392 - 402, XP055763657, ISSN: 1934-5909, DOI: 10.1016/j.stem.2013.09.006
- - ELENA REDONDO-CASTRO ET AL: "Interleukin-1 primes human mesenchymal stem cells towards an anti-inflammatory and pro-trophic phenotype in vitro", STEM CELL RESEARCH & THERAPY, vol. 8, no. 1, 17 April 2017 (2017-04-17), XP055583458, DOI: 10.1186/s13287-017-0531-4