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First published May 22, 2020 Application Aachen patent G – Physics

METHOD FOR MATRIX EFFECT CORRECTION IN QUANTITATIVE MASS SPECTROMETRIC ANALYSIS OF ANALYTES IN COMPLEX MATRICES

Espacenet Family 64902336

Abstract

The present invention relates to a method for quantifying one or more analytes in a sample by an analysis system comprising a separation unit (LC column), a means of adding a solution post-column (Connector), and a detection unit comprising a mass spectrometer coupled through an ionization source, the method comprising: • (i) inducing matrix effect on the analytes in the sample and on the post-column infused internal standards (PCI-ISs); • (ii) matching one or more post-column infused internal standard (PCI-IS) to each analyte that best matches the analyte's response to the matrix effect, and • (iii) storing the analyte-matched PCI-IS identification and, optionally, associated response data in a library; and • (iv) applying the analyte-matched PCI-IS to the analyte in other samples to correct the analyte peak responses for the matrix effect during ionization and to obtain (absolute) quantitation of the analyte using the response data.

Inventors 8

  1. Thomas Hankemeier Aachen author as filed: Hankemeier, Thomas
  2. Anne-Charlotte Dubbelman Aachen author as filed: Dubbelman, Anne Charlotte
  3. Amy C. Harms Aachen author as filed: Harms, Amy C.
  4. Oskar González Aachen author as filed: González, Oskar
  5. T·汉克迈耶
  6. A·c·杜贝尔曼
  7. O·冈萨雷斯
  8. Gonzalez, Orland

Inventors are linked to an Aachen author when surname and first name match exactly one author.

Applicants

Cited papers 7 in the database

Non-patent literature as cited (13)
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  • - OSKAR GONZÁLEZ 等: "Matrix Effect Compensation in Small-Molecule Profiling for an LC-TOF Platform Using Multicomponent Postcolumn Infusion", ANAL CHEM., vol. 87, no. 12, pages 5921 - 5929, XP055667800, DOI: 10.1021/ac504268y
  • - JULIA ROSSMANN 等: "Evaluation of the matrix effect of different sample matrices for 33 pharmaceuticals by post-column infusion", JOURNAL OF CHROMATOGRAPHY B, vol. 1000, pages 84 - 94, XP029257800, DOI: 10.1016/j.jchromb.2015.06.019
  • - 吴晟旻 等: "化学品测试项目的分析方法适用性研究", 2012中国环境科学学会学术年会论文集, vol. 4, 30 June 2012 (2012-06-30), pages 3376 - 3381

Cited patents 10

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