Combinational strategy for reducing ransom integration events when transfecting plants
Espacenet
Family 60937546
Abstract
The present disclosure provides methods for transfecting plants and for expressing RNA or polypeptide molecules in plants. In particular, plants having reduced expression and/or activity of a component of the classical NHEJ pathway and a component of the POLQ pathway are transfected in order to reduce random integration events. The disclosure further provides transfected plants and plant progeny produced by the methods disclosed herein.
Inventors 3
- Paul J. J. Hooykaas Aachen author as filed: Hooykaas, Paul Jan Jacob
- de Pater, Bernadette Sylvia
- Marcel Tijsterman Aachen author as filed: Tijsterman, Marcel
Inventors are linked to an Aachen author when surname and first name match exactly one author.
Applicants
- 莱顿大学
- Univ Leiden GBS Leiden
Cited papers 0 in the database
Non-patent literature as cited (2)
- - ALEX N. ZELENSKY等: "Inactivation of Pol θ and C-NHEJ eliminates off-target integration of exogenous DNA", 《NATURE COMMUNICATIONS》
- - AYAKO NISHIZAWA-YOKOI等: "Suppression of Ku70/80 or Lig4 leads to decreased stable transformation and enhanced homologous recombination in rice", 《NEW PHYTOLOGIST》